Document Detail

Forkhead (FOX) transcription factors and the cell cycle: measurement of DNA binding by FoxO and FoxM transcription factors.
MedLine Citation:
PMID:  15576937     Owner:  NLM     Status:  MEDLINE    
Certain forkhead (FOX) transcription factors have been shown to play an intrinsic role in controlling cell cycle progression. In particular, the FoxO subclass has been shown to regulate cell cycle entry and exit, whereas the expression and activity of FoxM1 is important for the correct coupling of DNA synthesis to mitosis. In this chapter, I describe a method for measuring FoxO and FoxM1 transcription factor DNA binding in nuclear extracts from mammalian cells.
Katrina A Bicknell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  296     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  247-62     Citation Subset:  IM    
School of Pharmacy, University of Reading, Reading, UK.
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MeSH Terms
Base Sequence
Binding Sites / genetics
Cell Cycle / physiology*
Cell Fractionation
Cell Line
Cell Nucleus / metabolism
DNA / genetics,  metabolism*
DNA-Binding Proteins / isolation & purification,  metabolism*
Electrophoretic Mobility Shift Assay / methods
Transcription Factors / isolation & purification,  metabolism*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Transcription Factors; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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