Document Detail

Forensic pregnancy diagnostics with placental mRNA markers.
MedLine Citation:
PMID:  19148664     Owner:  NLM     Status:  MEDLINE    
Current methods for pregnancy diagnostics are based on immunodetection of pregnancy-specific proteins and in a forensic context suffer from sensitivity and specificity issues. Here, we applied reverse transcriptase polymerase chain reaction (RT-PCR) technology to 11 genes previously reported with placental mRNA circulating in maternal blood. We found two genes, hPL and betahCG, with pregnancy-specific expression in whole blood samples. RT-PCR detection of hPL was positive in all samples tested throughout the pregnancy, whereas betahCG was detectable until half of the second trimester but not at later gestation ages. For hPL, in vitro stability of the transcript was demonstrated until 2 months of age, and the hPL-specific RT-PCR assay applied was highly sensitive with reliable detection from down to 0.25 cm(2) dried bloodstain. We therefore suggest hPL-specific RT-PCR as a new molecular tool for forensic pregnancy diagnostics from dried blood stains. Moreover, our results indicate that the time-wise reverse expression of hPL and betahCG during pregnancy may allow an RT-PCR-based estimation of the gestational age from blood stains, adding to the value of forensic pregnancy diagnosis for crime scene investigations.
Jeanot Gauvin; Dmitry Zubakov; Joke van Rhee-Binkhorst; Ate Kloosterman; Eric Steegers; Manfred Kayser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-16
Journal Detail:
Title:  International journal of legal medicine     Volume:  124     ISSN:  1437-1596     ISO Abbreviation:  Int. J. Legal Med.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-18     Completed Date:  2010-03-23     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9101456     Medline TA:  Int J Legal Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  13-7     Citation Subset:  IM    
Department of Forensic Molecular Biology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands.
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MeSH Terms
Blood Stains
Chorionic Gonadotropin, beta Subunit, Human / blood*,  genetics
Forensic Genetics
Genetic Markers*
Gestational Age
Placental Lactogen / blood*,  genetics
Pregnancy / blood*,  genetics
Pregnancy Trimesters
RNA, Messenger / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Chorionic Gonadotropin, beta Subunit, Human; 0/Genetic Markers; 0/RNA, Messenger; 9035-54-5/Placental Lactogen

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