Document Detail


Forelimb regeneration in the postmetamorphic bullfrog: stimulation by dimethyl sulfoxide and retinoic acid.
MedLine Citation:
PMID:  3489067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To examine the effects of retinoic acid and dimethyl sulfoxide on regenerative ability of anuran amphibians, the left forelimbs of 60 postmetamorphic froglets of Rana catesbeiana (bullfrogs) were amputated through the distal zeugopodium. Fifteen of the froglets had their left forelimb stumps immersed in dimethyl sulfoxide (DMSO) for 3 minutes, once immediately after amputation and once on each of 5 subsequent days. Another 15 frogs had their left forelimb stumps immersed in a 0.01 M solution of retinoic acid dissolved in DMSO for the same period of time. The remaining 30 control froglets did not regenerate structures distal to the amputation surface, while all limbs in both treated groups produced regenerates by 120 days postamputation. Regenerates of limbs treated with both DMSO alone and DMSO combined with retinoic acid, although hypomorphic, were composed of multiple cartilage elements, which in many cases (46.7%) were organized as patterns partially resembling the skeletal arrangement of a normal forelimb. All of these regenerates exhibited bundles of striated muscle. In addition, nearly half (46.7%) of the regenerates in the DMSO + retinoic acid group possessed two separate regenerate outgrowths. The results demonstrate that young bullfrogs (Rana catesbeiana) possess a latent epimorphic regenerative capability, which can be stimulated by topical application to the wound surface of DMSO alone or DMSO combined with retinoic acid.
Authors:
M L Cecil; R A Tassava
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  239     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-10-07     Completed Date:  1986-10-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dimethyl Sulfoxide / pharmacology*
Forelimb
Kinetics
Metamorphosis, Biological*
Rana catesbeiana
Regeneration / drug effects*
Time Factors
Tretinoin / pharmacology*
Grant Support
ID/Acronym/Agency:
NS-10165/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
302-79-4/Tretinoin; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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