Document Detail


Foreign protein production using plant cell and organ cultures: Advantages and limitations.
MedLine Citation:
PMID:  19463933     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants and plant tissue cultures are used as host systems for expression of foreign proteins including antibodies, vaccines and other therapeutic agents. Recombinant or stably transformed plants and plant cell cultures have been applied for foreign protein production for about 20 years. Because the product concentration achieved exerts a major influence on process economics, considerable efforts have been made by commercial and academic research groups to improve foreign protein expression levels. However, post-synthesis product losses due to protease activity within plant tissues and/or extracellular protein adsorption in plant cell cultures can negate the benefits of molecular or genetic enhancement of protein expression. Transient expression of foreign proteins using plant viral vectors is also a practical approach for producing foreign proteins in plants. Adaptation of this technology is required to allow infection and propagation of engineered viruses in plant tissue cultures for transient protein expression in vitro.
Authors:
Sharon M-H Shih; Pauline M Doran
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2009-05-20
Journal Detail:
Title:  Biotechnology advances     Volume:  27     ISSN:  1873-1899     ISO Abbreviation:  Biotechnol. Adv.     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-10-12     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8403708     Medline TA:  Biotechnol Adv     Country:  England    
Other Details:
Languages:  eng     Pagination:  1036-42     Citation Subset:  IM    
Affiliation:
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Plants / cytology,  metabolism*
Recombinant Proteins / biosynthesis*
Chemical
Reg. No./Substance:
0/Recombinant Proteins

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