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Forebrain GABAergic projections to locus coeruleus in mouse.
MedLine Citation:
PMID:  23296594     Owner:  NLM     Status:  Publisher    
The noradrenergic locus coeruleus (LC) regulates arousal, memory, sympathetic nervous system activity and pain. Forebrain projections to LC have been characterized in rat, cat and primates, but not systematically in mouse. We surveyed mouse forebrain LC projecting neurons by examining retrogradely labeled cells following LC iontophoresis of fluorogold and anterograde LC labeling after forebrain injection of biotinylated dextran-amine or viral tracer. Similar to other species, the central amygdalar nucleus (CAmy), anterior hypothalamus, paraventricular nucleus, and posterior lateral hypothalamic area (PLH) provide major LC inputs. Using mice expressing green fluorescent protein in GABAergic neurons we found that more than one-third of LC projecting CAmy and PLH neurons are GABAergic. LC colocalization of biotinylated dextran-amine, following CAmy or PLH injection, with either green fluorescent protein or GAD65/67-ir confirmed these GABAergic projections. CAmy injection of adeno-associated virus encoding channelrhodopsin-2-Venus showed similar fiber labeling and association with GAD65/67-ir and tyrosine hydroxylase-ir (TH-ir) neurons. CAmy and PLH projections were densest in a peri-coerulear zone, but many fibers entered the LC proper. Close apposition between CAmy GABAergic projections and TH-ir processes suggests CAmy GABAergic neurons may directly inhibit noradrenergic principal neurons. Direct LC neuron targeting was confirmed by anterograde transneuronal labeling of LC TH-ir neurons following CAmy or PLH injection of a herpes virus that expresses red fluorescent protein following activation by Cre recombinase in mice that express Cre recombinase in GABergic neurons. This description of GABAergic projections from CAmy and PLH to the LC clarifies important forebrain sources of inhibitory control of CNS noradrenergic activity. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
Eugene L Dimitrov; Yuchio Yanagawa; Ted B Usdin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  -     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Section on Fundamental Neuroscience, National Institute of Mental Health, 35 Convent Drive, Bethesda, MD 20892, USA.
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