| Forcing a connection: impacts of single-molecule force spectroscopy on in vivo tension sensing. | |
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MedLine Citation:
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PMID: 21267988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mechanical tension plays a large role in cell development ranging from morphology to gene expression. On the molecular level, the effects of tension can be seen in the dynamic arrangement of membrane proteins as well as the recruitment and activation of intracellular proteins. Forces applied to biopolymers during in vitro force measurements offer greater understanding of the effects of tension on molecules in live cells, and experimental techniques involving test tubes and live cells can often overlap. Indeed, when forces exerted on cellular components can be calibrated ex vivo with force spectroscopy, a powerful tool is available for researchers in probing cellular mechanotransduction on the molecular scale. This review will discuss the techniques used in measuring both cellular traction forces and single-molecule force spectroscopy. Emphasis will be placed on the use of fluorescence reporter systems for the development of in vivo tension sensors that can be used for calibration with single molecule force methods. |
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Authors:
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Michael D Brenner; Ruobo Zhou; Taekjip Ha |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review Date: 2011-01-25 |
Journal Detail:
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Title: Biopolymers Volume: 95 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-18 Completed Date: 2011-06-07 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 332-44 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biopolymers
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chemistry Elasticity Imaging Techniques / methods Fluorescence Resonance Energy Transfer / methods Magnetics / methods Mechanotransduction, Cellular Microscopy, Atomic Force / methods* Microscopy, Fluorescence / methods Optical Tweezers Stress, Mechanical |
| Grant Support | |
ID/Acronym/Agency:
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//Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Biopolymers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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