| Food stimulation of histidine decarboxylase messenger RNA abundance in rat gastric fundus. | |
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MedLine Citation:
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PMID: 8229845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Histidine decarboxylase in the enterochromaffin-like cells of the gastric corpus mucosa converts histidine to histamine which in turn stimulates gastric acid secretion. The control of histidine decarboxylase activity is poorly understood. We have examined how fasting and refeeding influence the abundance of the messenger RNA encoding histidine decarboxylase in the gastric corpus of the rat. 2. The polymerase chain reaction was used to generate a probe for detection of histidine decarboxylase messenger RNA in Northern and slot blots of total RNA from the gastric corpus of rats fasted for up to 48 h, or fasted and then refed. A gastrin monoclonal antibody was used to neutralize the action of endogenous gastrin. 3. Fasting progressively reduced histidine decarboxylase messenger RNA abundance by 3- to 4-fold after 48 h. Refeeding induced a rapid increase in histidine decarboxylase messenger RNA abundance which was detectable after 30 min. 4. There was a significant correlation between histidine decarboxylase messenger RNA abundance and plasma gastrin. Administration of gastrin antibody inhibited the increase in histidine decarboxylase activity after 6 h refeeding, but not after refeeding for 30 min. 5. The results suggest that histamine-mediated changes in postprandial acid secretion depend on control of histidine decarboxylase mRNA levels, and that gastrin regulates production of this enzyme in the rat over periods of a few hours. |
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Authors:
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R Dimaline; A K Sandvik; D Evans; E R Forster; G J Dockray |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of physiology Volume: 465 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-11-29 Completed Date: 1993-11-29 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 449-58 Citation Subset: IM |
Affiliation:
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MRC Secretory Control Research Group, Physiological Laboratory, University of Liverpool. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Female Food* Gastric Fundus / physiology* Gastrins / immunology, metabolism Histidine Decarboxylase / biosynthesis* Neutralization Tests RNA Probes / diagnostic use RNA, Messenger / biosynthesis* Rats Rats, Wistar |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Gastrins; 0/RNA Probes; 0/RNA, Messenger; EC 4.1.1.22/Histidine Decarboxylase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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