| Food restriction stimulates conjugation of p-nitrophenol in perfused rat liver. | |
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MedLine Citation:
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PMID: 7786027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rates of conjugation of p-nitrophenol were studied in livers from normal and food-restricted rats perfused with either p-nitroanisole or p-nitrophenol. Female Sprague-Dawley rats had ad libitum access to a Purina 5001 nonpurified diet (control) or were given 65% of the intake of controls for 3 weeks. Livers were perfused with oxygenated Krebs-Henseleit buffer using a nonrecirculating system. Maximal rates of conjugation of p-nitrophenol, generated either from the O-demethylation of p-nitroanisole (200 microM) or from the infusion of p-nitrophenol (70 microM), were elevated significantly nearly twofold by food restriction. Thus, food restriction stimulates conjugation in the intact liver cell. Specifically, rates of conjugation were increased from 2.1 +/- 0.2 to 3.7 +/- 0.4 and from 3.3 +/- 0.6 to 5.8 +/- 0.5 mumol/g/h when 200 microM p-nitroanisole or 70 microM p-nitrophenol were infused, respectively. On the other hand, rates of conjugation were not affected by food restriction when low concentrations of p-nitroanisole (50 microM) or p-nitrophenol (20 microM) were infused. Further, food restriction did not alter rates of conjugation in isolated microsomes supplemented with excess UDPGA. Interestingly, both UDP-glucose and UDP-glucuronic acid were increased significantly in liver extracts from food-restricted rats when livers were perfused with high but not low concentrations of p-nitrophenol. Under these conditions, the increase in UDP-glucuronic acid was threefold. Moreover, food restriction increased carbohydrate release from the liver about twofold. Glycogen content was also increased significantly in liver extracts from 8.4 +/- 1.9 to 60.4 +/- 13.8 mmol/kg wet weight by food restriction. Taken together, these data support the hypothesis that food restriction stimulates conjugation of p-nitrophenol concentrations by increasing the supply of the pivotal cofactor UDP-glucuronic acid from carbohydrate reserves (e.g., glycogen). |
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Authors:
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W Qu; F C Kauffman; R G Thurman |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 319 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-07-17 Completed Date: 1995-07-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 451-6 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of North Carolina at Chapel Hill 27599-7365, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrates / analysis Female Food Deprivation / physiology* Glucuronosyltransferase / analysis Liver / metabolism* Microsomes, Liver / metabolism Nitrophenols / metabolism* Perfusion Rats Rats, Sprague-Dawley Uridine Diphosphate Glucuronic Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Nitrophenols; 100-02-7/4-nitrophenol; 2616-64-0/Uridine Diphosphate Glucuronic Acid; EC 2.4.1.17/Glucuronosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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