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Food-borne enterococci integrate into oral biofilm: an in vivo study.
MedLine Citation:
PMID:  20951293     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
INTRODUCTION: Enterococci, particularly Enterococcus faecalis, are still a primary concern in endodontic infections. To date, enterococci have been considered to be only transiently present in the oral cavity. The aim of this study was to examine whether different enterococci from food are able to reside in oral biofilm.
METHODS: Six healthy volunteers wore dental splints loaded with enamel slabs. After 3 days, the volunteers consumed cheese containing enterococci. The fate of the enterococci was analyzed by culture technique and 16S rRNA gene sequencing. All isolates were characterized genotypically by macrorestriction analysis (SmaI) and pulsed-field gel electrophoresis. E. faecalis was also analyzed by using fluorescent in situ hybridization (FISH).
RESULTS: E. faecalis, E. faecium, E. avium, and E. durans were detected in the initial biofilm after 2 hours, as well as in the 5-day-old oral biofilm. E. faecalis, E. faecium, and E. avium isolated from the initial biofilm and from the 5-day-old biofilm, as well as those isolated from cheese, showed genetic homogeneity. E. faecium and E. avium had integrated into a pre-existing 3-day-old biofilm. No genetic similarity between E. durans strains isolated from cheese and those from the initial and 5-day-old oral biofilm was detected. E. faecalis was also detected in the oral biofilm by using FISH.
CONCLUSIONS: Food-borne enterococci, particularly E. faecalis, might not only be transient but could also survive in the oral biofilm and become a source for endodontic infections. Moreover, genotypic analysis is required to study the source of oral enterococci.
Authors:
Ali Al-Ahmad; Julia Maier; Marie Follo; Bettina Spitzmüller; Annette Wittmer; Elmar Hellwig; Johannes Hübner; Daniel Jonas
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Publication Detail:
Type:  Journal Article     Date:  2010-09-16
Journal Detail:
Title:  Journal of endodontics     Volume:  36     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1812-9     Citation Subset:  D    
Copyright Information:
Copyright © 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, Freiburg, Germany. ali.al-ahmad@uniklinik-freiburg.de
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