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Following healthy pregnancy by Nuclear Magnetic Resonance (NMR) metabolic profiling of human urine.
MedLine Citation:
PMID:  23231635     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this work, untargeted NMR metabonomics was employed to evaluate the effects of pregnancy on the metabolite composition of maternal urine, thus establishing a control excretory trajectory for healthy pregnancies. Urine was collected for independent groups of healthy non-pregnant and pregnant women (in 1(st), 2(nd), 3(rd) trimesters) and multivariate analysis performed on the corresponding NMR spectra. Models were validated through Monte Carlo Cross Validation and permutation tests and metabolite correlations measured through Statistical Total Correlation Spectroscopy. The levels of 21 metabolites were found to change significantly throughout pregnancy, with variations observed for the first time to our knowledge for choline, creatinine, 4-deoxyerythronic acid, 4-deoxythreonic acid, furoylglycine, guanidoacetate, 3-hydroxybutyrate and lactate. Results confirmed increased aminoaciduria across pregnancy and suggested a) a particular involvement of isoleucine and threonine in lipid oxidation/ketone body synthesis, b) a relation of excreted choline, taurine and guanidoacetate to methionine metabolism and urea cycle regulation and c) a possible relationship of furoylglycine and creatinine to pregnancy, based on a tandem study of non-fasting confounding effects. Results demonstrate the usefulness of untargeted metabonomics in finding biomarker metabolic signatures for healthy pregnancies, against which disease-related deviations may be confronted in future studies, as a base for improved diagnostics and prediction.
Authors:
Sílvia O Diaz; António S Barros; Brian J Goodfellow; Iola Fernandes Duarte; Isabel M Carreira; Eulália Galhano; Cristina Pita; Maria do Céu Almeida; Ana Maria Gil
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-11
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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