Document Detail


Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration.
MedLine Citation:
PMID:  23188412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Down-regulation with gonadodropin-releasing agonist (GnRH-a) protocol during IVF stimulation leads to a severe endogenous LH suppression, which may affect the follicular development. The aim of the study was to evaluate the effects of recombinant LH (r-LH) administration, during late follicular development stages, in recombinant FSH (r-FSH) stimulated cycles on follicular fluid (FF) parameters and on cumulus cell quality.
METHODS: Twenty patients undergoing IVF were stimulated in a long GnRH agonist protocol with r-FSH alone or with r-LH supplementation when the leading follicle reached diameter of 14 mm. FF was collected at the time of oocyte retrieval from 32 follicles ≥ 18 mm. Serum FSH, LH, estradiol (E(2)), and progesterone (P(4)) were evaluated on the day of hCG administration. Intra-follicular E(2), P(4), AMH and TGF-β were assayed. Total RNA from 18 individual cumuli was isolated for gene expression analyses.
RESULTS: R-LH increased FF P(4) levels. FF TGF-β levels and PTGS2 and HAS2 expression in cumulus cells (CCs) positively correlated with increased P(4) levels observed in FFs, while a negative correlation was found between P(4) and AMH levels.
CONCLUSIONS: FF positive correlation between P(4) and TGF-β levels and CC expression of PTGS2 and HAS2 suggest an association with a better follicle quality. In addition, our data suggest that late follicular phase r-LH supplementation leads to a more advanced stage of follicular maturation.
Authors:
Marzia Barberi; Beatrice Ermini; Maria Beatrice Morelli; Michele Ermini; Sandra Cecconi; Rita Canipari
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-28
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  2013-07-12     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1381-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cumulus Cells* / cytology,  drug effects,  metabolism
Down-Regulation
Estradiol / blood
Female
Fertilization in Vitro*
Follicle Stimulating Hormone / administration & dosage*,  blood,  genetics
Follicular Fluid* / drug effects,  secretion
Gene Expression Regulation, Developmental / drug effects
Gonadotropin-Releasing Hormone / administration & dosage*,  antagonists & inhibitors,  blood
Humans
Luteinizing Hormone / administration & dosage,  blood,  genetics
Oocytes / cytology,  drug effects
Ovarian Follicle / cytology,  metabolism
Ovulation Induction / methods
Pregnancy
Progesterone / administration & dosage,  blood
Recombinant Proteins / administration & dosage
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 33515-09-2/Gonadotropin-Releasing Hormone; 4G7DS2Q64Y/Progesterone; 4TI98Z838E/Estradiol; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone
Comments/Corrections

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