| Follicular dendritic cells inhibit apoptosis in human B lymphocytes by a rapid and irreversible blockade of preexisting endonuclease. | |
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MedLine Citation:
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PMID: 7759994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During germinal center reactions, a minority of B lymphocytes are selected after successful binding to follicular dendritic cells (FDCs). The majority of the B cells, however, die by apoptosis. One of the characteristics of apoptosis is rapid fragmentation of DNA by an endogenous endonuclease. The regulation of apoptosis and endonuclease activity in germinal center (GC) B cells is largely unknown. In this study we have investigated the induction and inhibition of endonuclease activity in GC B cells. We also investigated the role of FDCs, surface Ig (sIg), sIgM, CD21, CD22 CD40, and intracellular Zn2+ in the regulation of endonuclease activity. We have found that DNA fragmentation in GC B cells is caused by a preexisting endonuclease very similar to NUC-18 (an 18-kD endonuclease identified in rat thymocytes). Endonuclease activity in GC B cells appears to be rapidly and irreversibly blocked after interaction with FDCs, but not after cross-linkage of sIg, sIgM, CD21, CD22, or CD40. Addition of soluble CD40-human IgM fusion protein (sCD40) to FDC-B cell cultures also did not interfere with FDC-mediated B cell rescue. Chelation of intracellular Zn2+ during FDC-B cell cultures resulted in abrogated B cell rescue. These data suggest that FDCs inhibit apoptosis in GC B cells by a rapid inactivation of preexisting endonuclease using a mechanism distinct from CD40 ligation. |
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Authors:
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E Lindhout; A Lakeman; C de Groot |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 181 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-06-23 Completed Date: 1995-06-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1985-95 Citation Subset: IM |
Affiliation:
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Academic Medical Center, University of Amsterdam, Department of Cell Biology and Histology, The Netherlands. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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analysis Apoptosis / immunology* B-Lymphocyte Subsets / immunology, physiology B-Lymphocytes / immunology, physiology* Cell Communication Cell Separation Cells, Cultured Dendritic Cells / immunology* Endonucleases / antagonists & inhibitors*, isolation & purification, metabolism Humans Immunophenotyping Kinetics Palatine Tonsil / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; EC 3.1.-/Endonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 6 Year: 1995 Volume: 181 Issue: 6 First Page: 1985 Last Page: 1995 ID: 2192045 Publisher Id: 95279925 PubMed Id: 7759994 |
| Follicular dendritic cells inhibit apoptosis in human B lymphocytes by a rapid and irreversible blockade of preexisting endonuclease | |
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