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Folic acid uptake by the human syncytiotrophoblast is affected by gestational diabetes, hyperleptinemia, and TNF-α
MedLine Citation:
PMID:  23338599     Owner:  NLM     Status:  Publisher    
Background:The mechanisms whereby gestational diabetes mellitus (GDM) increases the risk of fetal overgrowth and development of metabolic diseases later in life are likely to involve changes in nutrient supply to the fetus. So, in this work we hypothesize that GDM may affect folic acid (FA) supply to the placenta and the fetus.Methods:We compared (3)H-FA uptake by human cytotrophoblasts isolated from normal pregnancies (NTB cells) and GDM pregnancies (DTB cells), and investigated the effect of GDM hallmarks upon (3)H-FA uptake by BeWo cells.Results:(3)H-FA uptake by NTB and DTB cells was time-dependent and acidic pH-stimulated. When compared to NTB, (3)H-FA uptake by DTB cells was more sensitive to acidic pH changes and to 5-methyltetrahydrofolate and pemetrexed inhibition, indicating a proportionally greater involvement of the proton-coupled folate transporter. A 4 h-exposure of BeWo cells to lipopolysaccharide (1-10 μg/ml) or to high levels of tumor necrosis factor-α (300 ng/l) significantly reduced (3)H-FA uptake. Moreover, hyperleptinemic conditions (100 ng/ml leptin) decreased (3)H-FA uptake by BeWo cells in a time-dependent manner, when compared to normoleptinemic conditions (1 ng/ml leptin).Conclusion:GDM modulates (3)H-FA uptake by the syncytiotrophoblast, and leptin as well as TNF-α downregulate (3)H-FA placental uptake.Pediatric Research (2013); doi:10.1038/pr.2013.14.
João R Araújo; Ana Correia-Branco; Liliana Moreira; Carla Ramalho; Fátima Martel; Elisa Keating
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Pediatric research     Volume:  -     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Porto, Portugal.
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