Document Detail


Folic acid-PEG conjugated superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI.
MedLine Citation:
PMID:  16736484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the development and in vitro study of a nanoconjugate serving as a targeted magnetic resonance imaging (MRI) contrast enhancement agent for detection of cancer cells overexpressing the folate receptor. The nanoconjugate was synthesized by coating superparamagnetic iron oxide nanoparticles with covalently bound bifunctional poly(ethylene glycol) (PEG), followed by conjugation with folic acid (FA). The specificity of the nanoconjugate targeting cancerous cells was demonstrated by comparative intracellular uptake of the nanoconjugate and PEG-/dextran-coated nanoparticles by human adenocarcinoma HeLa cells. Preferential targeting to cancerous cells was studied by comparing the uptake of the nanoconjugate by HeLa cells and by non-FR expressing osteosarcoma MG-63 cells. Uptake of the nanoconjugate by HeLa cells after 4 h incubation was found to be a 12-fold higher than that of PEG- or dextran-coated nanoparticles as quantified by inductively coupled plasma spectroscopy. A significant negative contrast enhancement was observed with magnetic resonance (MR) phantom imaging for HeLa cells over MG-63 cells, when both were cultured with the nanoconjugate. Specificity of the nanoconjugate for folate receptors was also verified with a competitive inhibition assay, in which HeLa cells were incubated with both NP-PEG-FA and free FA. The bifunctional PEG used has amide linkages within the PEG chains that can form interchain hydrogen bonding, leading to improved stability of the PEG coating. Self-assembled PEG can be controlled at the molecular level and are suitable for nanoscale coatings.
Authors:
Conroy Sun; Raymond Sze; Miqin Zhang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  78     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-25     Completed Date:  2006-12-27     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  550-7     Citation Subset:  IM    
Affiliation:
Department of Materials Science and Engineering, University of Washington, Seattle, 98195, USA.
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MeSH Terms
Descriptor/Qualifier:
Drug Delivery Systems*
Ferric Compounds
Folic Acid*
Hela Cells
Humans
Magnetic Resonance Imaging*
Magnetics
Nanostructures*
Polyethylene Glycols*
Grant Support
ID/Acronym/Agency:
N01-CO37122/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Ferric Compounds; 0/Polyethylene Glycols; 1309-37-1/ferric oxide; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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