Document Detail


Folic Acid Acts Through DNA Methyltransferases to Induce the Differentiation of Neural Stem Cells into Neurons.
MedLine Citation:
PMID:  23292356     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The present study investigated the roles of folic acid and DNA methyltransferases (DNMTs) in the differentiation of neural stem cells (NSCs). Neonatal rat NSCs were grown in suspended neurosphere cultures and identified by their expression of SOX2 protein and capacity for self-renewal. Then NSCs were assigned to five treatment groups for cell differentiation: control (folic acid-free differentiation medium), low folic acid (8 μg/mL), high folic acid (32 μg/mL), low folic acid and DNMT inhibitor zebularine (8 μg/mL folic acid and 150 nmol/mL zebularine), and high folic acid and zebularine (32 μg/mL folic acid and 150 nmol/mL zebularine). After 6 days of cell differentiation, immunocytochemistry and western blot analyses were performed to identify neurons by β-tubulin III protein expression and astrocytes by GFAP expression. We observed that folic acid increased DNMT activity which may be regulated by the cellular S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), and the abundance of neurons but decreased the number of astrocytes. Zebularine blocked these effects of folic acid. In conclusion, folic acid acts through elevation of DNMT activity to increase neuronal differentiation and decrease astrocytic differentiation in NSCs.
Authors:
Suhui Luo; Xumei Zhang; Min Yu; Hai Yan; Huan Liu; John X Wilson; Guowei Huang
Related Documents :
15064786 - The acid accelerated ruthenium-catalysed dihydroxylation. scope and limitations.
21658846 - Sulfur amino acid metabolism limits the growth of children living in environments of po...
17773776 - Lewis acidity of polar organic solvents from thermodynamic measurements.
11117876 - A 38 kda allylic alcohol dehydrogenase from the cultured cells of nicotiana tabacum.
6709136 - Cerebrospinal fluid ascorbic acid levels in neurological disorders.
418816 - Aminotransferases for aromatic amino acids and aspartate in bacillus subtilis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-6
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  -     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin, 300070, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Age-dependent effects of ELF-MF on oxidative stress in the brain of Mongolian gerbils.
Next Document:  Interaction of L-SIGN with Hepatitis C Virus Envelope Protein E2 Up-Regulates Raf-MEK-ERK Pathway.