| Folding nuclei of the scFv fragment of an antibody. | |
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MedLine Citation:
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PMID: 8679604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The folding kinetics of the variable domains of the phosphorylcholine-binding antibody McPC603, combined into a scFv fragment [VH-(Gly4Ser)3-VL], were investigated by the use of fluorescence spectroscopy, nuclear magnetic resonance (NMR), and mass spectrometry (MS). All three methods gave evidence for the occurrence of a major kinetic intermediate during the refolding of the denatured, oxidized scFv fragment. This intermediate is formed within the first 30 s of folding and comprises exchange-protected amide protons of hydrophobic and aromatic amino acids, most of which are localized within the inner beta-sheet of the V(L) domain. In the subsequent slow step, most of the amide protons become protected with rate constants that are very similar for residues of both domains. These data are in agreement with the MS results, which indicate a cooperative folding event from the intermediate to the native state of the scFv fragment. |
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Authors:
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C Freund; A Honegger; P Hunziker; T A Holak; A Plückthun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 35 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-20 Completed Date: 1996-08-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8457-64 Citation Subset: IM |
Affiliation:
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Biochemisches Institut, Universität Zürich, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antibodies / chemistry* Immunoglobulin Fab Fragments / chemistry* Immunoglobulin Variable Region / chemistry* Kinetics Magnetic Resonance Spectroscopy Mass Spectrometry Molecular Sequence Data Protein Conformation Protein Folding* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin Variable Region; 0/McPC603 antibody |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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