| Folate is related to phosphorylated neurofilament-H and P-tau (ser396) in rat brain. | |
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MedLine Citation:
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PMID: 21517845 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Protein phosphatase PP2A dephosphorylates phosphorylated tau (P-tau) and neurofilaments (pNFs). PP2A is S-adenosylmethionine (SAM)-dependent and might thus link methylation with neurodegeneration. Low SAM and increased S-adenosylhomocysteine (SAH) can enhance the risk of dementia. Methods: We studied the effect of hyperhomocysteinemia on P-tau(ser396), pNF-H (heavy chain), and PP2A-activity and level (the C subunit) in rat brain. Wistar rats (total n = 55) were fed either on a standard, a homocystine 1.7% or a methionine 2.4%- rich diet for 5 months. P-tau was tested in 21 frontal cortex tissue slices using immuno-fluorescence. Concentrations of pNF-H and the activity and level of PP2A were measured in brain extracts. Results: Concentrations of Hcy, SAM and SAH strongly increased in plasma of rats on the modified diets. The diets caused lowering of plasma folate and vitamin B12 and a significant increase in P-tau(ser396) in brain tissues but PP2A activity and level were unchanged. Plasma folate correlated to brain tissue PP2A activity (r = 0.28), pNF-H (r = -0.30), and P-tau (ser396) staining (r = -0.57) all p<0.05. Conclusions: Phosphorylation of brain functional proteins was related to folate. The effect of the diet on P-tau and pNF-H seemed not to be explained by a lower activity or protein level of PP2A. Folate might prove protective against multiple steps in the process of neurodegeneration. |
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Authors:
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Rima Obeid; Jennifer Schlundt; Natalia Umanskaya; Wolfgang Herrmann; Markus Herrmann |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-21 |
Journal Detail:
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Title: Journal of neurochemistry Volume: - ISSN: 1471-4159 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Journal of Neurochemistry © 2011 International Society for Neurochemistry. |
Affiliation:
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Department of Clinical Chemistry and Laboratory Medicine / Central Laboratory, University Hospital, Saarland University, 66424 Homburg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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