| Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy. | |
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MedLine Citation:
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PMID: 16089466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel method for the synthesis of highly monodispersed hydrophillic InP-ZnS nanocrystals and their use as luminescence probes for live cell imaging is reported. Hydrophobic InP-ZnS nanocrystals are prepared by a new method that yields high-quality, luminescent core-shell nanocrystals within 6-8 h of total reaction time. Then by carefully manipulating the surface of these passivated nanocrystals, aqueous dispersions of folate-conjugated nanocrystals (folate-QDs) with high photostability are prepared. By use of confocal microscopy, we demonstrate the receptor-mediated delivery of folic acid conjugated quantum dots into folate-receptor-positive cell lines such as KB cells. These folate-QDs tend to accumulate in multi-vescicular bodies of KB cells after 6 h of incubation. Receptor-mediated delivery was confirmed by comparison with the uptake of these particles in folate-receptor-negative cell lines such as A549. Efficient two-photon excitation of these particles and two-photon imaging using these particles are also demonstrated. The use of these InP-ZnS nanoparticles and their efficient two-photon excitation can be potentially useful for deep tissue imaging for future in vivo studies. |
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Authors:
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Dhruba J Bharali; Derrick W Lucey; Harishankar Jayakumar; Haridas E Pudavar; Paras N Prasad |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 127 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-10 Completed Date: 2005-11-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 11364-71 Citation Subset: IM |
Affiliation:
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Institute for Laser, Photonics and Biophotonics, and Department of Chemistry, The University of Buffalo, Buffalo, New York 14260-3000, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Carrier Proteins / metabolism* Cells, Cultured Fluorescent Dyes / chemical synthesis, chemistry*, metabolism* Humans Luminescent Agents / chemical synthesis, chemistry*, metabolism* Microscopy / methods Microscopy, Confocal* Microscopy, Fluorescence* Photons Quantum Dots* Receptors, Cell Surface / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Fluorescent Dyes; 0/Luminescent Agents; 0/Receptors, Cell Surface; 0/folate-binding protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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