Document Detail


Focused in vivo delivery of plasmid DNA to the porcine vascular wall via intravascular ultrasound destruction of microbubbles.
MedLine Citation:
PMID:  19923850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound.
OBJECTIVES/METHODS: The purpose of this study was to determine whether an intravascular ultrasound (IVUS) catheter could mediate plasmid DNA transfection from microbubble carriers to the porcine coronary artery wall following balloon angioplasty.
RESULTS: In the presence of plasmid-coupled microbubbles in vitro only cells exposed to ultrasound from the modified IVUS catheter significantly expressed the transgene. A porcine left anterior descending coronary artery underwent balloon angioplasty followed by injection and insonation of microbubbles from the IVUS catheter at the site of angioplasty. After 3 days, an approximately 6.5-fold increase in transgene expression was observed in arteries that received microbubbles and IVUS compared to those that received microbubbles with no IVUS.
CONCLUSIONS: The results of this study demonstrate for the first time that IVUS is required to enhance gene transfection from microbubble carriers to the vessel wall in vivo. This technology may be applied to both drug and gene therapy to reduce vessel restenosis.
Authors:
Linsey C Phillips; Alexander L Klibanov; Douglas K Bowles; Michael Ragosta; John A Hossack; Brian R Wamhoff
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-11-18
Journal Detail:
Title:  Journal of vascular research     Volume:  47     ISSN:  1423-0135     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-16     Completed Date:  2010-04-29     Revised Date:  2011-07-27    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  270-4     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
Biomedical Engineering, Robert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Angioplasty, Balloon, Coronary*
Animals
Cells, Cultured
Contrast Media / administration & dosage*
Coronary Vessels / metabolism*
Equipment Design
Luminescent Proteins / biosynthesis,  genetics
Microbubbles*
Microscopy, Fluorescence
Plasmids / metabolism*
Rats
Swine
Time Factors
Transfection / instrumentation,  methods*
Ultrasonography, Interventional* / instrumentation
Grant Support
ID/Acronym/Agency:
HL071574/HL/NHLBI NIH HHS; HL090700/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Luminescent Proteins; 0/red fluorescent protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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