Document Detail


The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment.
MedLine Citation:
PMID:  20173316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, there have been many reports concerning proteins which can recognize DNA double strand break (DSBs), and such proteins include histone H2AX phosphorylated at serine 139 (gammaH2AX), ataxia telangiectasia mutated (ATM) phospho-serine 1981, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) phospho-threonine 2609, Nijmegen breakage syndrome 1 (NBS1) phospho-serine 343, checkpoint kinase 2 (CHK2), phospho-threonine 68, and structural maintenance of chromosomes 1 (SMC1) phospho-serine 966. Thus, it should be possible to follow the formation of DSBs and their repair using immunohistochemical methods with multiple antibodies to detect these proteins. When normal human fibroblasts (AG1522 cells) were exposed to 3 Gy of X-rays as a control, clearly discernable foci for these proteins were detected, and these foci localized with gammaH2AX foci. After heat treatment at 45.5 degrees C for 20 min, these proteins are partially localized with gammaH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho-DNA-PKcs and phospho-CHK2) appeared at 8 h after heat treatment. These results are discussed from perspectives of heat-induced denaturation of proteins and formation of DSBs.
Authors:
Akihisa Takahashi; Eiichiro Mori; Takeo Ohnishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of radiation research     Volume:  51     ISSN:  1349-9157     ISO Abbreviation:  J. Radiat. Res.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-04     Completed Date:  2010-05-25     Revised Date:  2012-03-28    
Medline Journal Info:
Nlm Unique ID:  0376611     Medline TA:  J Radiat Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  91-5     Citation Subset:  IM    
Affiliation:
Department of Biology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
DNA / genetics,  radiation effects*
DNA Damage / physiology*
DNA-Binding Proteins / genetics,  metabolism*,  radiation effects*
Dose-Response Relationship, Radiation
Fibroblasts / physiology*,  radiation effects
Histones / genetics,  metabolism*,  radiation effects*
Hot Temperature
Humans
Radiation Dosage
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/H2AFX protein, human; 0/Histones; 9007-49-2/DNA

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