| Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results. | |
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MedLine Citation:
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PMID: 16230906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We sought to examine high-intensity focused ultrasound (HIFU)-induced hyperthermia in the liver of a rat model to focally induce green-fluorescent protein (GFP). MATERIALS AND METHODS: A total of 25 Copenhagen rats were included in this study. Rats were divided into groups treated with an adenovirus coding for green fluorescent protein (GFP) under the control of a hsp70B promoter and a CMV promoter. Ad-CMV-GFP-treated rats served as positive control. Untreated controls only subjected to MRI +/- HIFU-treatment served to find out optimal power of HIFU in the target area of the liver. Temperature was noninvasively monitored by temperature sensitive magnetic resonance imaging (MRI). RESULTS: Rats treated with Ad-hsp70B-GFP demonstrated localized gene induction within the liver parenchyma, in good correlation with MRI and histology. Applying an acoustic power of 1.92 W a relatively uniform focal temperature up to 42 +/- 5 degrees C within the liver parenchyma could be documented. 3 x 10(9) plaque-forming units proved to account for a very homogeneous liver infection. Number of fluorescent cells in the region of hyperthermia was similar to the control group treated with Ad-CMV-GFP. CONCLUSION: Using the introduced parameters spatially controlled gene induction within a parenchymal organ such as the liver in rats using HIFU under control of MRI is feasible. |
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Authors:
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Christian Plathow; Frank Lohr; Gabriela Divkovic; Guido Rademaker; Nabeel Farhan; Peter Peschke; Ivan Zuna; Juergen Debus; Claus D Claussen; Hans-Ulrich Kauczor; Chuan Yuan Li; Juergen Jenne; Peter Huber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative radiology Volume: 40 ISSN: 0020-9996 ISO Abbreviation: Invest Radiol Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-18 Completed Date: 2006-01-12 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0045377 Medline TA: Invest Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 729-35 Citation Subset: IM |
Affiliation:
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Department of Radiology, German Cancer Research Center Heidelberg, Germany. c.palthow@dkfz.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Gene Expression Regulation* Gene Therapy Genetic Vectors Green Fluorescent Proteins HSP70 Heat-Shock Proteins / genetics*, metabolism Heat-Shock Response / genetics* Hepatocytes / metabolism* Hyperthermia, Induced / methods* Liver / ultrasonography* Luminescent Agents Magnetic Resonance Imaging Male Microscopy, Fluorescence Models, Animal Promoter Regions, Genetic Rats Rats, Inbred Strains Transcriptional Activation Transgenes / physiology |
| Chemical | |
Reg. No./Substance:
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0/HSP70 Heat-Shock Proteins; 0/Luminescent Agents; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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