| Focal electroporation in zebrafish embryos and larvae. | |
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MedLine Citation:
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PMID: 19378103 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments. |
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Authors:
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Marcel Tawk; Isaac H Bianco; Jonathan D W Clarke |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 546 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-06-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 145-51 Citation Subset: IM |
Affiliation:
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MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House, 4th Floor, Guy's Hospital Campus, London SE1 1UL, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified / embryology, genetics, metabolism DNA / genetics, metabolism Developmental Biology / methods* Electroporation / methods* Embryo, Nonmammalian / cytology, drug effects Fluorescent Dyes / pharmacology Larva / cytology, drug effects Microscopy RNA / genetics, metabolism Rhodamines / pharmacology Time Factors Zebrafish / embryology*, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Rhodamines; 62669-72-1/tetramethylrhodamine; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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