| Focal adhesion kinase modulates cell adhesion strengthening via integrin activation. | |
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MedLine Citation:
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PMID: 19297531 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Focal adhesion kinase (FAK) is an essential nonreceptor tyrosine kinase regulating cell migration, adhesive signaling, and mechanosensing. Using FAK-null cells expressing FAK under an inducible promoter, we demonstrate that FAK regulates the time-dependent generation of adhesive forces. During the early stages of adhesion, FAK expression in FAK-null cells enhances integrin activation to promote integrin binding and, hence, the adhesion strengthening rate. Importantly, FAK expression regulated integrin activation, and talin was required for the FAK-dependent effects. A role for FAK in integrin activation was confirmed in human fibroblasts with knocked-down FAK expression. The FAK autophosphorylation Y397 site was required for the enhancements in adhesion strengthening and integrin-binding responses. This work demonstrates a novel role for FAK in integrin activation and the time-dependent generation of cell-ECM forces. |
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Authors:
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Kristin E Michael; David W Dumbauld; Kellie L Burns; Steven K Hanks; Andrés J García |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-03-18 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 20 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-01 Completed Date: 2009-07-20 Revised Date: 2011-04-01 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2508-19 Citation Subset: IM |
Affiliation:
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Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics / drug effects Cell Adhesion / drug effects Fibroblasts / cytology*, drug effects, enzymology* Fibronectins / metabolism Focal Adhesion Protein-Tyrosine Kinases / metabolism* Gene Knockdown Techniques Humans Integrin alpha5beta1 / metabolism* Integrins / metabolism* Kinetics Mice Phosphorylation / drug effects Phosphotyrosine / metabolism Protein Binding / drug effects Solubility / drug effects Talin / metabolism Tetracycline / pharmacology Vinculin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM065918-09/GM/NIGMS NIH HHS; R01-GM049882/GM/NIGMS NIH HHS; R01-GM065918/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 0/Integrin alpha5beta1; 0/Integrins; 0/Talin; 125361-02-6/Vinculin; 21820-51-9/Phosphotyrosine; 60-54-8/Tetracycline; EC 2.7.10.2/Focal Adhesion Protein-Tyrosine Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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