| Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1. | |
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MedLine Citation:
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PMID: 21087336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: Histopathological chorioamnionitis (HCA) is caused by microbial-driven infiltration of leukocytes to the maternal-fetal interface resulting in adverse neonatal outcomes in a subset of pregnancies. The role of placental villus macrophages (i.e. Hofbauer cells, HBCs) in the pathophysiology of HCA is unelucidated. METHOD OF STUDY: The number of HBCs in human term placental villi in HCA and control groups was compared using immunohistochemistry. Levels of monocyte chemotactic protein (MCP-1) expression were measured in primary cultures of syncytioytrophoblasts (SCTs) and fibroblasts (FIBs) treated with bacterial compounds [lipopolysaccharide (LPS) and peptidoglycan] and pro-inflammatory cytokines (TNF-α and IL-1β) using ELISA and quantitative real-time PCR. RESULTS: Immunohistochemistry revealed a focal increase in HBCs in HCA. Treatment of FIBs with LPS, IL-1β, and TNF-α significantly increased MCP-1 mRNA and protein expression. Conversely, MCP-1 mRNA and protein levels were virtually undetectable in treated and untreated SCTs. CONCLUSION: These results demonstrate cell-type-specific regulation of MCP-1 expression in human placenta. A model is presented in which bacterial products and inflammatory cytokines initiate a fibroblast-driven cytokine cascade resulting in recruitment of fetal monocytes to placenta which focally increases levels of HBCs in pregnancies complicated by HCA. |
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Authors:
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Paolo Toti; Felice Arcuri; Zhonghua Tang; Frederick Schatz; Eduardo Zambrano; Gil Mor; Tracy Niven-Fairchild; Vikki M Abrahams; Graciela Krikun; Charles J Lockwood; Seth Guller |
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Publication Detail:
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Type: Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural Date: 2010-11-19 |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 65 ISSN: 1600-0897 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-05 Completed Date: 2011-08-15 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 470-9 Citation Subset: IM |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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Department of Human Pathology & Oncology-Section of Pathology, University of Siena, Siena, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Chemokine CCL2 / metabolism* Chorioamnionitis / immunology*, pathology, physiopathology* Chorionic Villi / pathology Female Fetus / cytology, immunology* Fibroblasts / cytology, metabolism Humans Immunohistochemistry Lipopolysaccharides / immunology Macrophages / immunology* Placenta / cytology, immunology*, pathology Pregnancy Pregnancy Complications, Infectious / immunology, pathology, physiopathology |
| Grant Support | |
ID/Acronym/Agency:
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P01 HD054713-02/HD/NICHD NIH HHS; P01 HD054713-03/HD/NICHD NIH HHS; P01HD054713-01/HD/NICHD NIH HHS; R01 HD033909-13/HD/NICHD NIH HHS; R01HD33909-13/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Lipopolysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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