Document Detail


Focal high cell density generates a gradient of patterns in self-organizing vascular mesenchymal cells.
MedLine Citation:
PMID:  22797747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In embryogenesis, structural patterns, such as vascular branching, may form via a reaction-diffusion mechanism in which activator and inhibitor morphogens guide cells into periodic aggregates. We previously found that vascular mesenchymal cells (VMCs) spontaneously aggregate into nodular structures and that morphogen pairs regulate the aggregation into patterns of spots and stripes. To test the effect of a focal change in activator morphogen on VMC pattern formation, we created a focal zone of high cell density by plating a second VMC layer within a cloning ring over a confluent monolayer. After 24 h, the ring was removed and pattern formation monitored by phase-contrast microscopy. At days 2-8, the patterns progressed from uniform distributions to swirl, labyrinthine and spot patterns. Within the focal high-density zone (HDZ) and a narrow halo zone, cells aggregated into spot patterns, whilst in the outermost zone of the plate, cells formed a labyrinthine pattern. The area occupied by aggregates was significantly greater in the outermost zone than in the HDZ or halo. The rate of pattern progression within the HDZ increased as a function of its plating density. Thus, focal differences in cell density may drive pattern formation gradients in tissue architecture, such as vascular branching.
Authors:
Henry Cheng; Aneela Reddy; Andrew Sage; Jinxiu Lu; Alan Garfinkel; Yin Tintut; Linda L Demer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-11
Journal Detail:
Title:  Journal of vascular research     Volume:  49     ISSN:  1423-0135     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-27     Completed Date:  2012-10-31     Revised Date:  2013-07-15    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  441-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Medicine, University of California, Los Angeles, CA 90095-1679, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / embryology
Bone Morphogenetic Protein 2 / antagonists & inhibitors,  physiology
Cattle
Mesenchymal Stromal Cells / physiology*
Microscopy, Phase-Contrast
Morphogenesis / physiology*
Grant Support
ID/Acronym/Agency:
DK081346/DK/NIDDK NIH HHS; HL081202/HL/NHLBI NIH HHS; R01 DK081346/DK/NIDDK NIH HHS; R01 HL081202/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Protein 2
Comments/Corrections

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