Document Detail


Fnr mutants that activate gene expression in the presence of oxygen.
MedLine Citation:
PMID:  1898918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulatory protein Fnr is required for anaerobic expression of several anaerobic respiratory enzymes in Escherichia coli. To gain insight into how Fnr activity is regulated by oxygen, we have isolated Fnr mutants that increase expression of the nitrate reductase operon in the presence of oxygen (Fnr* mutants). Seven single-amino-acid substitutions that mapped within two regions of Fnr have been characterized. Two mutants mapped adjacent to two Cys residues in the N-terminal Cys cluster. Five Fnr* substitutions mapped to a region of Fnr that is similar to the cyclic AMP-binding domain of the catabolite activator protein (CAP). Within this group, four mutants were clustered in a region analogous to the CAP C helix, which is important in CAP dimer subunit interactions. Taken together, these data implicate regions in Fnr that may be important either in sensing oxygen deprivation or in the conformational change proposed to be necessary for Fnr activation under anaerobic conditions.
Authors:
P J Kiley; W S Reznikoff
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  173     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-02-20     Completed Date:  1991-02-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  16-22     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Allosteric Site
Amino Acid Sequence
Anaerobiosis
Bacterial Proteins / genetics*,  metabolism
Base Sequence
DNA, Bacterial / genetics,  isolation & purification
Escherichia coli / genetics*,  metabolism
Escherichia coli Proteins*
Gene Expression Regulation, Bacterial*
Genes, Bacterial*
Iron-Sulfur Proteins*
Molecular Sequence Data
Mutagenesis*
Nitrate Reductases / genetics
Operon
Plasmids
Restriction Mapping
Transcription Factors / genetics
beta-Galactosidase / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
GM12953/GM/NIGMS NIH HHS; GM19670/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Escherichia coli Proteins; 0/FNR protein, E coli; 0/Iron-Sulfur Proteins; 0/Transcription Factors; EC 1.7.-/Nitrate Reductases; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

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