Document Detail


Flux regulation in glycogen-induced oscillatory glycolysis in cell-free extracts of Saccharomyces carlsbergensis.
MedLine Citation:
PMID:  7082784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To localise the controlling point of the glycolytic system, the temporal changes in concentrations of glycolytic intermediates have been analysed after addition of glycogen to a substrate-depleted yeast extract. Three sequential metabolic states are clearly observable: a transition state at which there is continuous accumulation of the intermediates before the glyceraldehydephosphate dehydrogenase (GAPDH, EC 1.2.1.12) step; a stationary state with all glycolytic intermediates having concentrations oscillating at nearly stationary mean values; and a depletion state at which the intermediates before the GAPDH step are being depleted due to the exhaustion of glycogen. In all these states, the mean ethanol production rate and the concentration of ATP and the intermediates beyond the GAPDH-step are maintained fairly constant, while the glycogen consumption rate and intermediate concentrations of the upper part of the glycolytic system changes considerably: the glycogen consumption rate varies 4-fold and fructose-bis-phosphate concentration more than 10-fold. Doubling of the initial glycogen concentration and the addition of a great excess of fructose-bis-phosphate do not affect the ethanol production rate and the mean glycerate-3-phosphate (3-PGA) and pyruvate levels. By contrast, ethanol production was accelerated by an increase of the net ATP consumption rate resulting from either the addition of apyrase or by substitution of trehalose for glycogen. Neither the mean absolute ATP level nor the adenylate energy charge were measurably affected, however, all this data can be interpreted in terms of a very strong stoichiometric regulation and stabilization of the lower part of the glycolytic system.
Authors:
S B Jonnalagadda; J U Becker; E E Sel'kov; A Betz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bio Systems     Volume:  15     ISSN:  0303-2647     ISO Abbreviation:  BioSystems     Publication Date:  1982  
Date Detail:
Created Date:  1982-08-14     Completed Date:  1982-08-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0430773     Medline TA:  Biosystems     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  49-58     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Ethanol / metabolism
Fructosediphosphates / metabolism
Glucose-6-Phosphate
Glucosephosphates / metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
Glycogen / metabolism*,  pharmacology
Glycolysis* / drug effects
Kinetics
Saccharomyces / metabolism*
Subcellular Fractions / metabolism
Chemical
Reg. No./Substance:
0/Fructosediphosphates; 0/Glucosephosphates; 488-69-7/fructose-1,6-diphosphate; 56-65-5/Adenosine Triphosphate; 56-73-5/Glucose-6-Phosphate; 64-17-5/Ethanol; 9005-79-2/Glycogen; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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