| Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. | |
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MedLine Citation:
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PMID: 17284573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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White adipose tissue (WAT) mass is the main determinant of obesity and associated health risks. WAT expansion results from increases in white adipocyte cell number and size, which in turn reflect a series of shifts in the cellular metabolic state. To quantitatively profile the metabolic alterations occurring during de novo adipocyte formation, metabolic flux analysis (MFA) was used in conjunction with a novel modularity analysis algorithm on differentiating 3T3-L1 preadipocytes. Use of a type I collagen gel as an effective long-term culture substrate was also assessed. The calculated flux distributions predicted the sequential activation of several intracellular cross-compartmental pathways, including lipogenesis, the pentose phosphate pathway, and the malate cycle, in good agreement with earlier isotopic tracer experiments and gene profiling studies. Partition of the adipocyte metabolic network into highly interacting reaction subgroups suggested a functional reorganization of the major pathways consistent with the lipid-loading phenotype of the adipocyte. Flux and modularity analysis results together point to the flux distribution around pyruvate as a key indicator of adipocyte lipid accumulation. |
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Authors:
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Yaguang Si; Jeongah Yoon; Kyongbum Lee |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-02-06 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 292 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-05 Completed Date: 2007-07-30 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1637-46 Citation Subset: IM |
Affiliation:
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Department of Biology, Tufts University, Medford, MA 02155, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells
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cytology* Adipocytes, White / cytology*, metabolism* Algorithms Animals Cell Differentiation / drug effects, physiology* Cell Proliferation Collagen Type I / pharmacology DNA / metabolism Gels Lipid Metabolism Lipogenesis Malates / metabolism Mice Pentose Phosphate Pathway Phenotype |
| Grant Support | |
ID/Acronym/Agency:
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1-R21-DK-67228-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Gels; 0/Malates; 6915-15-7/malic acid; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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