Document Detail


Flux balance analysis of mixed microbial cultures: Application to the production of polyhydroxyalkanoates from complex mixtures of volatile fatty acids.
MedLine Citation:
PMID:  23036926     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fermented agro-industrial wastes are potential low cost substrates for polyhydroxyalkanoates (PHA) production by mixed microbial cultures (MMC). The use of complex substrates has however profound implications in the PHA metabolism. In this paper we investigate PHA accumulation using a lumped metabolic model that describes PHA storage from arbitrary mixtures of volatile fatty acids (VFA). Experiments were conducted using synthetic and complex VFA mixtures obtained from the fermentation of sugar cane molasses. Metabolic flux analysis (MFA) and flux balance analysis (FBA) were performed at different stages of culture enrichment in order to investigate the effect of VFA composition and time of enrichment in PHA storage efficiency. Substrate uptake and PHA storage fluxes increased over enrichment time by 70% and 73%, respectively. MFA calculations show that higher PHA storage fluxes are associated to an increase in the uptake of VFA with even number of carbon atoms and a more effective synthesis of hydroxyvalerate (HV) precursors from VFA with odd number of carbons. Furthermore, FBA shows that the key metabolic objective of a MMC subjected to the feast and famine regimen is the minimization of the tricarboxylic acid cycle fluxes. The PHA flux and biopolymer composition (hydroxybutyrate (HB): HV) could be accurately predicted in several independent experiments.
Authors:
Filipa Pardelha; Maria G E Albuquerque; Maria A M Reis; João M L Dias; Rui Oliveira
Related Documents :
23082086 - Management of diabetes and its complications with banaba (lagerstroemia speciosa l.) an...
24139156 - Isotope fractionation and spectroscopic analysis as an evidence of cr(vi) reduction dur...
24422636 - Tetramic acid analogues produced by coculture of saccharopolyspora erythraea with fusar...
23053236 - Sensory complementation and antipredator behavioural compensation in acid-impacted juve...
18840136 - Sorafenib inhibits mapk-mediated proliferation in a barrett's esophageal adenocarcinoma...
12482456 - Cytotoxic triterpenes from the twigs of celtis philippinensis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Journal of biotechnology     Volume:  -     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains...
Next Document:  Production and Characterization of a Novel Human Recombinant Alpha-1-Antitrypsin in PER.C6 cells.