| Fluorouracil selectively enriches stem-like cells in the lung adenocarcinoma cell line SPC. | |
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MedLine Citation:
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PMID: 23359275 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Most adult stem cells are in the G0 or quiescent phase of the cell cycle and account for only a small percentage of the cells in the tissue. Thus, isolation of stem cells from tissues for further study represents a major challenge. This study sought to enrich cancer stem cells and explore cancer stem-like cell clones using 5-fluorouracil (5-FU) in the lung adenocarcinoma cell line, SPC. Proliferation inhibition was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, according to which half maximal inhibitory concentration values were calculated. Expression levels of stem cell markers after treatment with 5-FU were examined using immunofluorescence and Western blotting. Additionally, side population (SP) cells were sorted using FACS. Properties of SP cells were evaluated by using Transwell, colony-forming assays, and tumor formation experiments. 5-FU greatly inhibits proliferation, especially of cells in S phase. SP cells possess greater invasive potential, higher clone-forming potential, and greater tumor-forming ability than non-SP cells. Treatment with 5-FU enriches the SP cells with stem cell properties in human lung adenocarcinoma cell lines. |
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Authors:
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Mu-Mu Shi; Yan-Lei Xiong; Xin-Shan Jia; Xin Li; Li Zhang; Xiao-Lei Li; En-Hua Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-29 |
Journal Detail:
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Title: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine Volume: - ISSN: 1423-0380 ISO Abbreviation: Tumour Biol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8409922 Medline TA: Tumour Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Pathology, The First Affiliated Hospital of China Medical University, Shenyang, China, shimumu2009@139.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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