Document Detail


Fluorometric measurement of light absorption by the rabbit cornea.
MedLine Citation:
PMID:  7523159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bench specular microscope, modified to perform as a scanning microfluorometer, was used to make simple and precise measurements of the absorption of visible light by the isolated rabbit cornea. The tissue was scanned while identical FITC dextran solutions were perfused over both the surfaces, and the measured fluorescence levels in the solutions on the two sides were compared. The alteration in level corresponds to the difference between the absorption of the cornea and that of of an equal thickness of the solution, which was determined in a separate experiment. The absorption of light at 500 nm ranged from 0-8% with a mean of 3.3% (n = 20; S.E. = 0.52). Pigmented and non-pigmented rabbits did not show any clear difference in absorption. These results suggests that light absorption is not a major source of error in anterior segment fluorophotometry.
Authors:
D M Maurice; S P Srinivas
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental eye research     Volume:  58     ISSN:  0014-4835     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-10-21     Completed Date:  1994-10-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  409-13     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Stanford University, CA 94305.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Animals
Cornea / physiology*
Dextrans
Fluorescein-5-isothiocyanate / analogs & derivatives
Fluorescence
Fluorometry
Light*
Rabbits
Scattering, Radiation
Grant Support
ID/Acronym/Agency:
EY00431/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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