Document Detail


Fluorometric determination of chemically available lysine: adaptation, validation and application to different milk products.
MedLine Citation:
PMID:  14727768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A spectrophotometric method based on the reaction between available lysine and ortho-phthaldialdehyde (OPA) was adapted and validated for fluorometric determination of the chemically available lysine contents in milk matrices (UHT and conventional in-bottle sterilized cow milk, milk-based infant formulas and infant formula ingredients). The values of the analytical parameters show its usefulness as a routine method (linearity, r = 0.9992; detection limit, 0.0066 mg/mL assay; accuracy, 99-108%; precision, intra-day 2.1-5.9% and inter-day 3.5 10.2%). No statistically significant differences (p < 0.05) were found between the values obtained with the adapted method and those obtained applying the 1-fluoro-2,4-dinitrobenzene (FDNB) (Carpenter) technique. The OPA method was used to measure the chemically available lysine contents in UHT and sterilized milk marketed in Spain, to study the evolution of chemically available lysine during the shelf-life of UHT milks, and finally the quality of name- and store-brand UHT milks was also compared. No statistically significant differences (p < 0.05) were found between either the available lysine contents of the same type of UHT or sterilized milk or between store- and name-brand UHT milks. Statistically significant differences (p < 0.05) were found between the chemically available lysine contents in UHT and sterilized milk. Losses of chemically available lysine ranging from 2.7 to 29% were obtained during the shelf-life of UHT milk.
Authors:
E Ferrer; A Alegría; Rosaura Farré; P Abellán; F Romero
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Validation Studies    
Journal Detail:
Title:  Die Nahrung     Volume:  47     ISSN:  0027-769X     ISO Abbreviation:  Nahrung     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-03-08     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0142530     Medline TA:  Nahrung     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  403-7     Citation Subset:  IM    
Affiliation:
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, E-46100 Burjassot, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Availability
Calibration
Fluorometry / methods*
Food Handling / methods
Humans
Infant
Infant Food / analysis
Infant, Newborn
Lysine / analysis*
Milk / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
56-87-1/Lysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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