| Fluorometric detection of deoxyribonucleic acid synthesis; possibilities for interfacing bromodeoxyuridine dye techniques with flow fluorometry. | |
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MedLine Citation:
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PMID: 70459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorometric detection of the biosynthetic incorporation of 5-bromodeoxyuridine (BrdU) into deoxyribonucleic acid has permitted cytologic studies of chromosome structure, replication, and repair. Some of these phenomena, previously detected using BrdU-dye techniques on fixed microscopic preparations, should be particularly amenable to analogous experimentation in fluorescence flow systems. Problems involved in interfacing BrdU-dye methodology with flow fluorometry are discussed. The effects of certain chemical modifications of bisbenzimidazole dyes on their spectroscopic properties and potential use for detecting BrdU incorporation into unfixed cells are described. Data on the use and energy transfer characteristics of a pair of deoxyribonucleic acid-binding dyes (33258 Hoechst and ethidium bromide) capable of simultaneously providing information about BrdU substitution and total deoxyribonucleic acid content are presented. |
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Authors:
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S A Latt |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 25 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1977 Jul |
Date Detail:
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Created Date: 1977-10-28 Completed Date: 1977-10-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 913-26 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bisbenzimidazole
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diagnostic use Bromodeoxyuridine / metabolism* Cell Line Chromatids / physiology Crossing Over, Genetic DNA / biosynthesis* Ethidium / diagnostic use Fluorescent Dyes / diagnostic use Sex Chromosomes / metabolism Spectrometry, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 23491-44-3/Bisbenzimidazole; 3546-21-2/Ethidium; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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