| Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues. | |
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MedLine Citation:
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PMID: 1063412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range. |
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Authors:
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K A Gruber; S Stein; L Brink; A Radhakrishnan; S Udenfriend |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 73 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1976 Apr |
Date Detail:
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Created Date: 1976-07-06 Completed Date: 1976-07-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1314-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chromatography / methods Fluorescent Dyes Male Oxytocin / analysis* Peptides / isolation & purification Pituitary Gland / analysis* Rats Vasopressins / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Fluorescent Dyes; 0/Peptides; 11000-17-2/Vasopressins; 50-56-6/Oxytocin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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