Document Detail


Fluorocarbon perfusion medium applied to the isolated rat brain.
MedLine Citation:
PMID:  7453195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to overcome the disadvantages of erythrocytes and albumin, a totally synthetic medium for the perfusion of the isolated rat brain was developed. the perfusion medium was based upon Krebs-Henseleit solution and contained in addition a fluorocarbon (FC 43) for oxygen transport and a polyol (Pluronic F 68) to furnish emulsifying and oncotic properties. The physico-chemical properties of this fluorocarbon perfusion medium allowed perfusion of an isolated rat brain to continue for more than 7 hr, while the medium could be stored as a stock emulsion for several months. The appropriate function of the isolated perfused rat brain is demonstrated by the EEG and by various substrates of the energy metabolism. The suitability of the fluorocarbon medium for perfusion of an isolated rat brain preparation is discussed.
Authors:
B Dirks; J Krieglstein; H H Lind; H Rieger; H Schütz
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological methods     Volume:  4     ISSN:  0160-5402     ISO Abbreviation:  J Pharmacol Methods     Publication Date:  1980 Sep 
Date Detail:
Created Date:  1981-03-17     Completed Date:  1981-03-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7806596     Medline TA:  J Pharmacol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  95-108     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Substitutes / pharmacology*
Brain / metabolism,  physiology*
Chemical Phenomena
Chemistry
Electroencephalography
Energy Metabolism
Fluorocarbons / pharmacology*
Glucose / metabolism
Male
Perfusion*
Rats
Chemical
Reg. No./Substance:
0/Blood Substitutes; 0/Fluorocarbons; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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