Document Detail


Fluorinated xerogel-derived microelectrodes for amperometric nitric oxide sensing.
MedLine Citation:
PMID:  18714964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An amperometric fluorinated xerogel-derived nitric oxide (NO) microelectrode is described. A range of fluorine-modified xerogel polymers were synthesized via the cohydrolysis and condensation of alkylalkoxy- and fluoroalkoxysilanes. Such polymers were evaluated as NO sensor membranes to identify the optimum composition for maximizing NO permeability while providing sufficient selectivity for NO in the presence of common interfering species. By taking advantage of both the versatility of sol-gel chemistry and the "poly(tetrafluoroethylene)-like" high NO permselective properties of the xerogels, the performance of the fluorinated xerogel-derived sensors was excellent, surpassing all miniaturized NO sensors reported to date. In contrast to previous electrochemical NO sensor designs, xerogel-based NO microsensors were fabricated using a simple, reliable dip-coating procedure. An optimal permselective membrane was achieved by synthesizing xerogels of methyltrimethoxysilane (MTMOS) and 20% (heptadecafluoro-1,1,2,2-tetrahydrodecyl)trimethoxysilane (17FTMS, balance MTMOS) under acid-catalyzed conditions. The resulting NO microelectrode had a conical tip of approximately 20 microm in diameter and approximately 55 microm in length and exhibited sensitivities of 7.91 pA x nM (-1) from 0.2 to 3.0 nM (R (2) = 0.9947) and 7.60 nA x microM (-1) from 0.5 to 4.0 microM ( R (2) = 0.9999), detection limit of 83 pM (S/ N = 3), response time ( t 95%) of <3 s, and selectivity (log K NO, j (amp)) of -5.74, <-6, <-6, <-6, <-6, -5.84, and -1.33 for j = nitrite, ascorbic acid, uric acid, acetaminophen, dopamine, ammonia/ammonium, and carbon monoxide. In addition, the sensor proved functional up to 20 d, maintaining >or=90% of the sensor's initial sensitivity without serious deterioration in selectivity.
Authors:
Jae Ho Shin; Benjamin J Privett; Justin M Kita; R Mark Wightman; Mark H Schoenfisch
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-08-20
Journal Detail:
Title:  Analytical chemistry     Volume:  80     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2008-10-30     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6850-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chemistry Techniques, Analytical / instrumentation*
Electrochemistry
Fluorine / chemistry*
Gels / chemistry*
Membranes, Artificial
Microelectrodes
Nitric Oxide / analysis*
Permeability
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
DA10900/DA/NIDA NIH HHS; EB000708/EB/NIBIB NIH HHS; R01 DA010900/DA/NIDA NIH HHS; R01 DA010900-10/DA/NIDA NIH HHS; R01 DA010900-11/DA/NIDA NIH HHS; R01 DA010900-12/DA/NIDA NIH HHS; R01 EB000708/EB/NIBIB NIH HHS; R01 EB000708-05/EB/NIBIB NIH HHS; R01 EB000708-06A1/EB/NIBIB NIH HHS; R01 EB000708-07/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Gels; 0/Membranes, Artificial; 284SYP0193/Fluorine; 31C4KY9ESH/Nitric Oxide
Comments/Corrections

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