| Fluorinated alpha-methylamino acids as 19F NMR indicators of intracellular pH. | |
| | |
MedLine Citation:
|
PMID: 6626667 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A series of nontoxic, fluorinated amino acids and their methyl esters have been used to measure intracellular pH in human peripheral blood lymphocytes by 19F NMR. Mono-, di-, and trifluoro alpha-methylalanines and their methyl esters have been characterized with respect to their spectra, the pH dependence of their fluorine resonances, and the effects of temperature and cations on the NH2pKa values. These pH indicators can be used singly or in combination to determine intracellular vs. extracellular pH profiles over a wide range (greater than 2 pH units) of extracellular pH. |
| | |
Authors:
|
J S Taylor; C Deutsch |
Related Documents
:
|
19362467 - Quaternary ammonium n,n-dichloroamines as topical, antimicrobial agents. 11542077 - Oligoglyceric acid synthesis by autocondensation of glyceroyl thioester. 10791737 - Metabolism of lactococcus lactis subsp. cremoris mg 1363 in acid stress conditions. 6527127 - Growing hyphae of achlya bisexualis generate a longitudinal ph gradient in the surround... 16658277 - Indoleacetic acid and abscisic acid antagonism: i. on the phytochrome-mediated attachme... 15767547 - Boswellic acid acetate induces apoptosis through caspase-mediated pathways in myeloid l... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biophysical journal Volume: 43 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1983 Sep |
Date Detail:
|
Created Date: 1983-12-17 Completed Date: 1983-12-17 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 261-7 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alanine
/
analogs & derivatives*,
blood Humans Hydrogen-Ion Concentration Kinetics Lymphocytes / metabolism* Magnetic Resonance Spectroscopy / methods Temperature |
| Grant Support | |
ID/Acronym/Agency:
|
AM 00838/AM/NIADDK NIH HHS; AM 27595/AM/NIADDK NIH HHS; GM 12202/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
56-41-7/Alanine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heritability: one word, three concepts.
Next Document: Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene.