| Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids. | |
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MedLine Citation:
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PMID: 5418479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The isolation of microamounts (5-30 micro g) of individual glycosphingolipids from a mixture, and their quantitative estimation is described. The percentage composition of a glycosphingolipid mixture from the kidneys of adult C57/BL male mice is given. |
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Authors:
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L Coles; G M Gray |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of lipid research Volume: 11 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1970 Mar |
Date Detail:
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Created Date: 1970-04-27 Completed Date: 1970-04-27 Revised Date: 2003-12-29 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 164-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Alcohols
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analysis* Animals Cerebrosides / analysis Chromatography, Thin Layer Fluorescence Fluorometry Glycolipids / analysis* Kidney / analysis* Lung / analysis Male Methods Mice Naphthalenes Sulfonic Acids Swine |
| Chemical | |
Reg. No./Substance:
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0/Amino Alcohols; 0/Cerebrosides; 0/Glycolipids; 0/Naphthalenes; 0/Sulfonic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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