| Fluorimetric determination of intra- and extracellular free amino acids in the microalgae Tetraselmis gracilis (Prasinophyceae) using monolithic column in reversed phase mode. | |
| | |
MedLine Citation:
|
PMID: 19569093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This paper describes the development and application of an RP HPLC method using a C(18) monolithic stationary phase for the separation and quantification of extra- and intracellular amino acids in a batch cultivation of the marine alga Tetraselmis gracilis. Fluorimetric detection was made after separation of the o-phthaldialdehyde 2-mercaptoethanol (OPA-2MCE) derivatives using a binary gradient elution. Separation of 19 amino acids was achieved with resolution >1.5 in about 39 min at a flow rate of 1.5 mL/min. RSD of analyses in seawater medium ranged from 0.36% for Orn (0.50 micromol/L) to 12% for Ile (0.10 micromol/L). The main constituents of the intracellular dissolved free amino acids (DFAAs) in the exponential growth phase were arginine (Arg), asparagine (Asn), alanine (Ala), aspartic acid (Asp), glutamic acid (Glu), serine (Ser), glycine (Gly), glutamine (Gln), and leucine (Leu). The major amino acids excreted to the media were valine (Val), Ala, Ser, and Gly. The monolithic phase facilitates the analysis by shortening the separation time and saving solvents and instrumentation costs (indeed conventional HPLC instrumentation can be used, running at lower pressures than those ones used with packed particle columns). |
| | |
Authors:
|
José Carlo Pires Penteado; Marilda Rigobello-Masini; Cleber Wanderlei Liria; M Terêsa Machini Miranda; Jorge Cesar Masini |
Related Documents
:
|
9719553 - Human globin chain separation by capillary electrophoresis in acidic isoelectric buffers. 3578753 - Determination of methylated basic, 5-hydroxylysine, elastin crosslinks, other amino aci... 3355593 - Microsomal cytochrome p-452 induction and peroxisome proliferation by hypolipidaemic ag... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of separation science Volume: 32 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-17 Completed Date: 2009-10-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
|
Languages: eng Pagination: 2827-34 Citation Subset: IM |
Affiliation:
|
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Algae, Green*
/
chemistry,
genetics Amino Acids / analysis* Anion Exchange Resins / chemistry Chromatography, High Pressure Liquid Chromatography, Ion Exchange Fluorometry / instrumentation, methods* Seawater |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Anion Exchange Resins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intrinsic Activity and Poisoning Rate for HCOOH Oxidation at Pt(100) and Vicinal Surfaces Containing...
Next Document: Preparation and characterization of microporous fibers for sample preparation and LC-MS determinatio...