| Fluorimetric determination of ascorbic acid in vitamin C tablets using methylene blue. | |
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MedLine Citation:
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PMID: 16204979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, a simple and sensitive fluorimetric method was described for the determination of Ascorbic Acid (AA). The procedure is based on the reaction between AA and Methylene Blue (MB). The fluorescence intensity of MB was measured at excitation and emission of 664 and 682 nm, respectively. MB concentration was decreased as a function of decreasing fluorescence intensity due to forming colorless form of MB (Leuco-MB) in the reaction between AA and MB. A linear relationship was obtained between the decreasing fluorescence intensity and the concentration of AA in the range of 3.0 x 10(-7)-6.0 x 10(-6) mol.l(-1). The detection limit was 2.5 x 10(-7) mol.l(-1). The proposed method was applied successfully for the determination of AA in Vitamin C tablets. |
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Authors:
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Yusuf Dilgin; Gürel Nişli |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemical & pharmaceutical bulletin Volume: 53 ISSN: 0009-2363 ISO Abbreviation: Chem. Pharm. Bull. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-05 Completed Date: 2006-02-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0377775 Medline TA: Chem Pharm Bull (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 1251-4 Citation Subset: IM |
Affiliation:
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Ege University, Science Faculty, Department of Chemistry. ydilgin@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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analysis*,
chemistry Calibration Methylene Blue / chemistry* Molecular Structure Sensitivity and Specificity Spectrometry, Fluorescence / methods Tablets / chemistry Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Tablets; 50-81-7/Ascorbic Acid; 61-73-4/Methylene Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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