Document Detail


Fluorimetric determination of ascorbic acid in vitamin C tablets using methylene blue.
MedLine Citation:
PMID:  16204979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, a simple and sensitive fluorimetric method was described for the determination of Ascorbic Acid (AA). The procedure is based on the reaction between AA and Methylene Blue (MB). The fluorescence intensity of MB was measured at excitation and emission of 664 and 682 nm, respectively. MB concentration was decreased as a function of decreasing fluorescence intensity due to forming colorless form of MB (Leuco-MB) in the reaction between AA and MB. A linear relationship was obtained between the decreasing fluorescence intensity and the concentration of AA in the range of 3.0 x 10(-7)-6.0 x 10(-6) mol.l(-1). The detection limit was 2.5 x 10(-7) mol.l(-1). The proposed method was applied successfully for the determination of AA in Vitamin C tablets.
Authors:
Yusuf Dilgin; Gürel Nişli
Related Documents :
2768269 - Ascorbic acid within chromaffin granules. in situ kinetics of norepinephrine biosynthesis.
6162499 - Effect of variation in endogenous levels of ascorbic acid on the in vitro immunological...
9873419 - Ascorbic acid-based inhibitors of alpha-amylases.
9722529 - Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase.
11368779 - Intracellular ascorbic acid enhances the dna single-strand breakage and toxicity induce...
15209539 - Capacity of ascorbyl palmitate to produce the ascorbyl radical in vitro: an electron sp...
1212229 - Tissue distribution of retinol and its metabolites after administration of double-label...
15170119 - Novel fusicoccins r and s, and the fusicoccin s aglycon (phomopsiol) from phomopsis amy...
10845379 - Microextraction of nine haloacetic acids in drinking water at microgram per liter level...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  53     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2006-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1251-4     Citation Subset:  IM    
Affiliation:
Ege University, Science Faculty, Department of Chemistry. ydilgin@yahoo.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analysis*,  chemistry
Calibration
Methylene Blue / chemistry*
Molecular Structure
Sensitivity and Specificity
Spectrometry, Fluorescence / methods
Tablets / chemistry
Time Factors
Chemical
Reg. No./Substance:
0/Tablets; 50-81-7/Ascorbic Acid; 61-73-4/Methylene Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synthesis of ibuprofen eugenol ester and its microemulsion formulation for parenteral delivery.
Next Document:  Biologically active glycosides from Asteroidea, 41. Isolation and structure determination of glucoce...