Document Detail

Fluorimetric determination of ascorbic acid in vitamin C tablets using methylene blue.
MedLine Citation:
PMID:  16204979     Owner:  NLM     Status:  MEDLINE    
In this study, a simple and sensitive fluorimetric method was described for the determination of Ascorbic Acid (AA). The procedure is based on the reaction between AA and Methylene Blue (MB). The fluorescence intensity of MB was measured at excitation and emission of 664 and 682 nm, respectively. MB concentration was decreased as a function of decreasing fluorescence intensity due to forming colorless form of MB (Leuco-MB) in the reaction between AA and MB. A linear relationship was obtained between the decreasing fluorescence intensity and the concentration of AA in the range of 3.0 x 10(-7)-6.0 x 10(-6) mol.l(-1). The detection limit was 2.5 x 10(-7) mol.l(-1). The proposed method was applied successfully for the determination of AA in Vitamin C tablets.
Yusuf Dilgin; Gürel Nişli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  53     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2006-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1251-4     Citation Subset:  IM    
Ege University, Science Faculty, Department of Chemistry.
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MeSH Terms
Ascorbic Acid / analysis*,  chemistry
Methylene Blue / chemistry*
Molecular Structure
Sensitivity and Specificity
Spectrometry, Fluorescence / methods
Tablets / chemistry
Time Factors
Reg. No./Substance:
0/Tablets; 50-81-7/Ascorbic Acid; 61-73-4/Methylene Blue

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