| Fluorimetric determination of D-amino acid oxidase. | |
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MedLine Citation:
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PMID: 4382256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. A sensitive fluorimetric procedure for the assay of d-amino acid oxidase has been developed. 2. The method depends on the formation of a fluorescent derivative, 2-hydroxy-3-methylquinoxaline, on condensation of pyruvate with o-phenylenediamine in acid medium. 3. 2-Hydroxy-3-methylquinoxaline fluoresces strongly in 50% (v/v) sulphuric acid after excitation at 375mmu. A single emission peak is observed at 480mmu. 4. Formation of the quinoxaline is dependent on time, temperature, acidity and relative concentration of reactants. 5. A particulate preparation from mouse kidney required FAD for optimum activity at pH8.5; K(m) was 3.8x10(-3)m; K(FAD) was 1.4x10(-7)m and the reaction was strongly inhibited by p-chloromercuribenzoate and phenylmercuric acetate. 6. Subcellular fractionation on a sucrose density gradient confirmed that the d-amino acid oxidase was localized on small granules. |
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Authors:
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M A Verity; R Gallagher; W J Brown |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 103 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1967 May |
Date Detail:
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Created Date: 1967-10-04 Completed Date: 1967-10-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 375-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aniline Compounds Animals Centrifugation, Density Gradient Chloromercuribenzoates D-Amino-Acid Oxidase / analysis* Enzymes Flavin-Adenine Dinucleotide Fluorometry* Hydrogen-Ion Concentration Kidney / enzymology Kinetics Mercury Mice Pyruvates Quinoxalines / analysis Temperature |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Chloromercuribenzoates; 0/Enzymes; 0/Pyruvates; 0/Quinoxalines; 146-14-5/Flavin-Adenine Dinucleotide; 7439-97-6/Mercury; EC 1.4.3.3/D-Amino-Acid Oxidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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