Document Detail


Fluorimetric determination of D-amino acid oxidase.
MedLine Citation:
PMID:  4382256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. A sensitive fluorimetric procedure for the assay of d-amino acid oxidase has been developed. 2. The method depends on the formation of a fluorescent derivative, 2-hydroxy-3-methylquinoxaline, on condensation of pyruvate with o-phenylenediamine in acid medium. 3. 2-Hydroxy-3-methylquinoxaline fluoresces strongly in 50% (v/v) sulphuric acid after excitation at 375mmu. A single emission peak is observed at 480mmu. 4. Formation of the quinoxaline is dependent on time, temperature, acidity and relative concentration of reactants. 5. A particulate preparation from mouse kidney required FAD for optimum activity at pH8.5; K(m) was 3.8x10(-3)m; K(FAD) was 1.4x10(-7)m and the reaction was strongly inhibited by p-chloromercuribenzoate and phenylmercuric acetate. 6. Subcellular fractionation on a sucrose density gradient confirmed that the d-amino acid oxidase was localized on small granules.
Authors:
M A Verity; R Gallagher; W J Brown
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  103     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1967 May 
Date Detail:
Created Date:  1967-10-04     Completed Date:  1967-10-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aniline Compounds
Animals
Centrifugation, Density Gradient
Chloromercuribenzoates
D-Amino-Acid Oxidase / analysis*
Enzymes
Flavin-Adenine Dinucleotide
Fluorometry*
Hydrogen-Ion Concentration
Kidney / enzymology
Kinetics
Mercury
Mice
Pyruvates
Quinoxalines / analysis
Temperature
Chemical
Reg. No./Substance:
0/Aniline Compounds; 0/Chloromercuribenzoates; 0/Enzymes; 0/Pyruvates; 0/Quinoxalines; 146-14-5/Flavin-Adenine Dinucleotide; 7439-97-6/Mercury; EC 1.4.3.3/D-Amino-Acid Oxidase
Comments/Corrections

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