Document Detail

Fluorimetric detection of serum corticosterone using high-performance liquid chromatography.
MedLine Citation:
PMID:  1452617     Owner:  NLM     Status:  MEDLINE    
A simple, sensitive, specific and reproducible method for the determination of corticosterone concentrations in rat serum using high-performance liquid chromatography (HPLC) with fluorimetric detection is described. Corticosterone is detectable down to 0.1 ng injected onto the HPLC column. Cortisol is used as an internal standard. Ethyl acetate was used for both initial serum corticosteroid extraction and the subsequent fluorophore extraction after sulfuric acid hydrolysis; thus sulfuric acid does not enter the HPLC system. The resultant fluorophores for both corticosterone and cortisol are stable for at least two weeks at ambient temperature not requiring storage at -20 degrees C. The procedure is highly suitable for use with HPLC systems utilising automatic sample injectors. The method is specific for corticosterone; dexamethasone, cortisone and gonadal steroids are not detectable and do not interfere in this assay.
S R Mason; L C Ward; P E Reilly
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  581     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-01-06     Completed Date:  1993-01-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  267-71     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Corticosterone / blood*
Rats, Wistar
Spectrometry, Fluorescence
Stress, Physiological
Reg. No./Substance:

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