Document Detail

Fluoride, triclosan and organic weak acids as modulators of the arginine deiminase system in biofilms and suspension cells of oral streptococci.
MedLine Citation:
PMID:  19572886     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: The arginine deiminase system (ADS) of oral bacteria is a major generator of alkali (ammonia) in dental plaque and is considered to have anticaries effects. However, many of the antimicrobial agents used in oral care products may reduce alkali production by the ADS. The objective of our work was to assess the sensitivity of the ADS of oral streptococci to commonly used antimicrobials, fluoride, triclosan and organic weak acids. METHODS: Streptococcus sanguinis NCTC 10904 and Streptococcus ratti FA-1 were grown in suspension cultures and mono-organism biofilms. ADS activity at pH values of 4, 5 and 6 was assessed, and the actions of the agents was determined in terms of reduced production of alkali from arginine, inhibition of ADS enzymes and changes in uptake of arginine. RESULTS: ADS activity was not greatly affected by pH changes between 4 and 6 and was greater per unit of biomass for cell suspensions than for biofilms. NaF was a poor inhibitor, while triclosan was highly effective with a 50% inhibitory dose for the two organisms between 0.03 and 0.05 and between 0.10 and 0.15 mm-h for suspension cells and biofilms, respectively. The weak acid indomethacin was nearly as potent at pH 4.0 as triclosan, while capric and lauric acids were less potent, especially for biofilms. The methyl ester of lauric acid was slightly stimulatory. The major targets for the inhibitors appeared to be transport systems for arginine uptake, although carbamate kinase was a secondary target. CONCLUSION: Triclosan, indomethacin, caprate and laurate can reduce ADS activity in dental plaque.
E Barboza-Silva; A C D Castro; R E Marquis
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Oral microbiology and immunology     Volume:  24     ISSN:  1399-302X     ISO Abbreviation:  Oral Microbiol. Immunol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-03     Completed Date:  2009-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707451     Medline TA:  Oral Microbiol Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  265-71     Citation Subset:  D    
Department of Microbiology and Immunology and Center for Oral Biology, University of Rochester Medical Center, Rochester, NY, USA.
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MeSH Terms
Anti-Infective Agents, Local / pharmacology*
Arginine / metabolism
Biofilms / drug effects
Biological Transport / drug effects
Cariostatic Agents / pharmacology
Decanoic Acids / pharmacology
Dental Plaque / enzymology,  microbiology*
Hydrogen-Ion Concentration
Hydrolases / antagonists & inhibitors*,  metabolism
Indomethacin / pharmacology*
Laurates / pharmacology
Lauric Acids / pharmacology
Phosphotransferases (Carboxyl Group Acceptor) / antagonists & inhibitors
Sodium Fluoride / pharmacology
Streptococcus / drug effects*,  enzymology*
Triclosan / pharmacology*
Grant Support
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Cariostatic Agents; 0/Decanoic Acids; 0/Laurates; 0/Lauric Acids; 111-82-0/lauric acid methyl ester; 143-07-7/lauric acid; 334-48-5/decanoic acid; 3380-34-5/Triclosan; 53-86-1/Indomethacin; 74-79-3/Arginine; 7681-49-4/Sodium Fluoride; EC 2.7.2.-/Phosphotransferases (Carboxyl Group Acceptor); EC kinase; EC 3.-/Hydrolases; EC deiminase

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