Document Detail

Fluoride-treated bio-resorbable synthetic nonceramic [corrected] hydroxyapatite promotes proliferation and differentiation of human osteoblastic MG-63 cells.
MedLine Citation:
PMID:  22107042     Owner:  NLM     Status:  MEDLINE    
When resorbable hydroxyapatite (HA) granules, which are used as a bone supplement material, were treated in neutral 4% sodium fluoride (NaF) solution, formation of a reactant resembling calcium fluoride was observed on the surface of the granules. Immediate and slow release of fluoride from fluoridated HA (HA+F) granules was observed after immersion in culture fluid, and the concentration increased over time to 1.25 ± 0.05 ppm F at 0.5 hours, 1.57 ± 0.12 ppm F at 24 hours, and 1.73 ± 0.15 ppm F at 48 hours. On invasion assay, migration of human osteoblast-like MG-63 cells exposed to the released fluoride was confirmed in comparison to the cells incubated with a nonfluoridated control sample (P < .01). In addition, fluoride added to the medium increased MG-63 cell proliferation in a manner dependent on fluoride concentrations up to 2.0 ppm (P < .05). At 5.0 ppm, however, fluoride significantly inhibited cell proliferation (P < .005). Activity of the osteogenic differentiation marker, alkaline phosphatase (ALP), also increased with fluoride after exposure for 1 week, increasing significantly at 1.0 ppm (P < .05). The promotion of MG-63 cell migration and proliferation, as well as increased ALP activity, suggested that fluoride released from the surface of resorbable HA granules, which were fluoridated by prior treatment with neutral 4% NaF solution, can provide a superb method to supply fluoride and promote osteogenic cell differentiation.
Motofumi Ohno; Kazunari Kimoto; Toshihisa Toyoda; Kazushige Kawata; Hirohisa Arakawa
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2011-11-22
Journal Detail:
Title:  The Journal of oral implantology     Volume:  39     ISSN:  0160-6972     ISO Abbreviation:  J Oral Implantol     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-24     Completed Date:  2013-07-12     Revised Date:  2013-09-12    
Medline Journal Info:
Nlm Unique ID:  7801086     Medline TA:  J Oral Implantol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  154-60     Citation Subset:  D    
Department of Health Science, Kanagawa Dental College, Yokosuka, Japan.
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MeSH Terms
Absorbable Implants*
Alkaline Phosphatase / drug effects
Cell Line
Cell Movement / drug effects
Cell Proliferation / drug effects
Culture Media
Delayed-Action Preparations
Dose-Response Relationship, Drug
Durapatite / chemistry,  pharmacology*
Fluorides / analysis,  chemistry,  pharmacology*
Hydroxyapatites / chemistry,  pharmacology
Ion-Selective Electrodes
Materials Testing
Osteoblasts / drug effects*
Osteogenesis / drug effects
Sodium Fluoride / chemistry
Time Factors
Reg. No./Substance:
0/Culture Media; 0/Delayed-Action Preparations; 0/Fluorides; 0/Hydroxyapatites; 0/fluorohydroxyapatite; 1306-06-5/Durapatite; 7681-49-4/Sodium Fluoride; EC Phosphatase
Comment In:
J Oral Implantol. 2013 Apr;39(2):234-5   [PMID:  23611679 ]
Erratum In:
J Oral Implantol. 2013 Aug;39(4):415

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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