Document Detail


Fluorescent study of chromatin and tubulin in apparently unfertilized human oocytes following ICSI.
MedLine Citation:
PMID:  9872363     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we examined 138 oocytes which were meiotically mature and, on light microscopic examination, contained either no or one pronucleus following intracytoplasmic sperm injection (ICSI). Oocytes were fixed and simultaneously stained for chromatin (Hoechst 33258) and the spindle (alpha-tubulin antibody). In nine oocytes, no sperm nucleus was observed. The remaining oocytes were separated into two groups following staining; (i) oocytes which had remained at metaphase II after ICSI (n = 74); and (ii) oocytes in which resumption of meiosis was observed after ICSI (n = 55). In all oocytes in which sperm chromatin was absent no resumption of meiosis had occurred and therefore parthenogenetic activation by the process of ICSI seems to be a rare event. In 17 out of 74 (23%) oocytes which remained at metaphase II, staining identified premature chromosome condensation (PCC) of the sperm chromatin (G1-PCC). Sperm nuclear decondensation or further transformation of the sperm chromatin was observed in 56 out of 74 (76%) oocytes which remained at metaphase II after ICSI and in 46 out of 55 (84%) oocytes which had resumed meiosis, indicating that initiation of sperm decondensation is independent of the resumption of meiosis in the oocyte. In contrast, transition of the sperm nucleus beyond the decondensed stage only occurred in association with resumption of meiosis in the oocyte (no pronuclei in metaphase II oocytes). The presence of both male and female pronuclei in 53% of oocytes which had resumed meiosis indicates that changes in sperm chromatin beyond the initial decondensation stage are dependent on cytoplasmic conditions which also permit female pronuclear formation.
Authors:
D Gook; S M Osborn; H Bourne; D H Edgar; A L Speirs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular human reproduction     Volume:  4     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-12     Completed Date:  1999-03-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1130-5     Citation Subset:  IM    
Affiliation:
Reproductive Biology Unit, Royal Women's Hospital, Carlton, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / chemistry
Chromatin / chemistry,  physiology*
Cytoplasm
Female
Fertilization in Vitro / methods*
Humans
Male
Meiosis
Metaphase
Microinjections
Microscopy, Fluorescence
Oocytes / cytology,  physiology*
Spermatozoa / chemistry,  pathology,  physiology
Tubulin / chemistry,  physiology*
Chemical
Reg. No./Substance:
0/Chromatin; 0/Tubulin

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