| Fluorescent retrograde axonal tracing of the facial nerve. | |
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MedLine Citation:
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PMID: 17003730 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The objective of this study was to investigate retrograde fluorescent axonal labeling of motor nerves as an aid to surgical dissection in otolaryngology-head and neck surgery. METHODS: Cholera toxin B subunit conjugated to a fluorescent dye was injected into the facial musculature of New Zealand white rabbits. At variable time points after injection, the facial nerves were harvested and analyzed for fluorescence in the peripheral axon. RESULTS: All injected animals demonstrated axonal fluorescence. No animal demonstrated facial weakness. Fluorescence was detected a maximum of 20 mm from the distal end of the nerve. Time periods greater than 48 hours did not substantially increase transport distance. CONCLUSIONS: Cholera toxin B subunit fluorescent conjugates, when administered intramuscularly, reliably label the distal portion of the facial nerve. In vivo labeling of a motor nerve may have significant potential for identifying a nerve in surgery. This work represents a preliminary investigation into the adaptation of in vivo fluorescence techniques as an aid to surgical dissection. Further research to refine this technique should be supported. |
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Authors:
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Matthew R O'Malley; Justin E Wittkopf; Jeffrey L Cutler; Robert F Labadie; Troy A Hackett; David S Haynes |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Laryngoscope Volume: 116 ISSN: 0023-852X ISO Abbreviation: Laryngoscope Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-03 Completed Date: 2006-10-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8607378 Medline TA: Laryngoscope Country: United States |
Other Details:
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Languages: eng Pagination: 1792-7 Citation Subset: IM |
Affiliation:
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Vanderbilt University Department of Hearing and Speech Sciences, Nashville, Tennessee, USA. matthew.o.malley@vanderbilt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axons / ultrastructure* Cholera Toxin / diagnostic use Facial Muscles / innervation Facial Nerve / anatomy & histology* Female Fluorescent Dyes / diagnostic use* Image Processing, Computer-Assisted Injections, Intramuscular Male Microscopy, Fluorescence Motor Neurons / cytology* Neural Pathways / anatomy & histology Rabbits Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 9012-63-9/Cholera Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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