Document Detail


Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells.
MedLine Citation:
PMID:  20188954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging.
Authors:
Jing Zhang; Xing Jia; Xiao-Jing Lv; Yu-Lin Deng; Hai-Yan Xie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-29
Journal Detail:
Title:  Talanta     Volume:  81     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  505-9     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier B.V. All rights reserved.
Affiliation:
School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aptamers, Nucleotide / genetics,  metabolism*
Base Sequence
Biocompatible Materials / chemistry,  metabolism
Biotin / metabolism
Cell Line, Tumor
Fluorescent Dyes / chemistry*,  metabolism*
Kinetics
Liver Neoplasms / metabolism,  pathology*
Mice
Molecular Probes / chemistry*,  metabolism*
Quantum Dots*
Reproducibility of Results
Staining and Labeling
Streptavidin / metabolism
Substrate Specificity
Temperature
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Biocompatible Materials; 0/Fluorescent Dyes; 0/Molecular Probes; 58-85-5/Biotin; 9013-20-1/Streptavidin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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