| Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells. | |
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MedLine Citation:
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PMID: 20188954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging. |
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Authors:
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Jing Zhang; Xing Jia; Xiao-Jing Lv; Yu-Lin Deng; Hai-Yan Xie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-29 |
Journal Detail:
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Title: Talanta Volume: 81 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 505-9 Citation Subset: IM |
Copyright Information:
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(c) 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aptamers, Nucleotide / genetics, metabolism* Base Sequence Biocompatible Materials / chemistry, metabolism Biotin / metabolism Cell Line, Tumor Fluorescent Dyes / chemistry*, metabolism* Kinetics Liver Neoplasms / metabolism, pathology* Mice Molecular Probes / chemistry*, metabolism* Quantum Dots* Reproducibility of Results Staining and Labeling Streptavidin / metabolism Substrate Specificity Temperature |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/Biocompatible Materials; 0/Fluorescent Dyes; 0/Molecular Probes; 58-85-5/Biotin; 9013-20-1/Streptavidin |
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