Document Detail

Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells.
MedLine Citation:
PMID:  20188954     Owner:  NLM     Status:  MEDLINE    
Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging.
Jing Zhang; Xing Jia; Xiao-Jing Lv; Yu-Lin Deng; Hai-Yan Xie
Related Documents :
21055744 - Cell selection by selective matrix adhesion is not sufficiently efficient for complete ...
3494684 - Effect of growth factors on the proliferation of thymocytes in different functional and...
21051144 - Kinetics of cell lysis for microcystis aeruginosa and nitzschia palea in the exposure t...
3369274 - Differentiation of adreno-chromaffin cells in the newborn rat, as detected by formaldeh...
15523894 - 1-amino-4-phenyl-1,2,3,6-tetrahydropyridine and 1-amino-4-phenylpyridinium salt, the 1-...
18667864 - Mutanase induction in trichoderma harzianum by cell wall of laetiporus sulphureus and i...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-29
Journal Detail:
Title:  Talanta     Volume:  81     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  505-9     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier B.V. All rights reserved.
School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aptamers, Nucleotide / genetics,  metabolism*
Base Sequence
Biocompatible Materials / chemistry,  metabolism
Biotin / metabolism
Cell Line, Tumor
Fluorescent Dyes / chemistry*,  metabolism*
Liver Neoplasms / metabolism,  pathology*
Molecular Probes / chemistry*,  metabolism*
Quantum Dots*
Reproducibility of Results
Staining and Labeling
Streptavidin / metabolism
Substrate Specificity
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Biocompatible Materials; 0/Fluorescent Dyes; 0/Molecular Probes; 58-85-5/Biotin; 9013-20-1/Streptavidin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A novel chemiluminescence method for the determination of ergometrine maleate in serum sample withou...
Next Document:  Carbon paste and PVC electrodes for the flow injection potentiometric determination of dextromethorp...