| Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 22534822 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli. |
| | |
Authors:
|
Tomás García-Cayuela; Luz P Gómez de Cadiñanos; M Luz Mohedano; Pilar Fernández de Palencia; Daniel Boden; Jerry Wells; Carmen Peláez; Paloma López; Teresa Requena |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-27 |
Journal Detail:
|
Title: Applied microbiology and biotechnology Volume: - ISSN: 1432-0614 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), CEI UAM+CSIC, Nicolás Cabrera 9, 28049, Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of ozone on the biodegradation of 17?-ethinylestradiol and sulfamethoxazole by mixed bact...
Next Document: Biochemical analysis of a highly specific, pH stable xylanase gene identified from a bovine rumen-de...