Document Detail

Fluorescent probes of chromosome structure and replication.
MedLine Citation:
PMID:  76502     Owner:  NLM     Status:  MEDLINE    
Procedures employing fluorescent dyes or Giemsa stain have been utilized to differentiate methaphase chromosomes into longitudinal segments termed bands. In spite of the immense practical utility of chromosome banding, the chemical basis of banding patterns remains incompletely understood. Physical chemical studies have elucidated the modes and specificities of the interaction of fluorescent dyes such as quinacrine, 33258 Hoechst, daunomycin, chromomycin A3 and 7-aminoactinomycin D with DNA and chromatin. However, it is not clear that all aspects of chromosome staining are explainable in terms of the optical properties of soluble dye-DNA complexes. BrdU-dye techniques in which chromosome staining depends on the schedule of BrdU incorporation by cells, have been used for cytological studies of chromosome structure and replication. These procedures have revealed a close association between quinacrine or Giemsa bands and late replicating chromosomal regions. Biochemical studies on chromatin differentially labelled according to replication timing may thus prove useful for investigating the molecular basis of chromosome banding.
S A Latt
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie     Volume:  19     ISSN:  0008-4093     ISO Abbreviation:  Can. J. Genet. Cytol.     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-06-12     Completed Date:  1978-06-12     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0372706     Medline TA:  Can J Genet Cytol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  603-23     Citation Subset:  IM    
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MeSH Terms
Azure Stains
Base Sequence
Chromosomes / ultrastructure*
Clostridium perfringens / genetics
DNA / analysis,  genetics,  metabolism
DNA Replication
Drosophila / genetics
Fluorescent Dyes*
Staining and Labeling*
Reg. No./Substance:
0/Azure Stains; 0/Fluorescent Dyes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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