Document Detail


Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique.
MedLine Citation:
PMID:  7653638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) were injected. Two FM and two RM labels were injected simultaneously to enable inter- and intramethod comparison. After gamma-counting samples of blood, myocardium (n = 168), and other organs (n = 59) were digested in test tubes with 2 N ethanolic KOH (60 degrees C, 48 h), microspheres were sedimented by centrifugation, dye was extracted in the same tube, and fluorescence was measured. With this processing method, recovery of FM was approximately 100%. Good correlations for inter- and intramethod comparisons were found [r = 0.985 +/- 0.01 (mean +/- SD)]. The lower intermethod correlation for blue microspheres (r = 0.958) indicates that the use of this label is less desirable. RM and FM endocardial-to-epicardial blood flow ratios correlated well (r = 0.974). With this one-vessel centrifugal sedimentation method and at least five fluorescently labeled microspheres, blood flow can be reliably measured in various organs, including ischemic myocardium.
Authors:
M F Van Oosterhout; H M Willigers; R S Reneman; F W Prinzen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-22     Completed Date:  1995-09-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H725-33     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Limburg, Maastricht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Circulation*
Coronary Disease / physiopathology*
Dogs
Female
Fluorescence
Histological Techniques
Male
Microspheres

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